A comparative study of antioxidant active components in different parts of Artemisia argyi by UPLC-ABTS-Q-TOF-MS
In order to characterize and identify the chemical components in different parts of Artemisia argyi(roots,stems,leaves,and seeds),compounds with antioxidant activity were screened.In this study,ultra-performance liquid chromatography-2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt-quadrupole time-of-flight-tandem mass spectrometry(UPLC-ABTS-Q-TOF-MS)was used as an online combination technique.Poroshell 120 SB-Aq(3.0 mm×150 mm,2.7 μm)was used as the column,and acetonitrile(A)-0.2%formic acid water(B)was adopted as the mobile phase to perform gradient elution and was scanned in positive and negative ion modes.MassLynx software was utilized,and combined with reference substances and related literature,the chemical components of different parts of A.argyi were identified and compared.The antioxidant active components were detected by using the online detection system,and the antioxidant activities of active components of different parts of A.argyi were compared and evaluated by scavenging efficiency.As a result,a total of 87 compounds were identified from extracts of different parts of A.argyi,and 38,72,85,and 33 components were identified from roots,stems,leaves,and seeds.22 compounds with antioxidant activity were screened,and 14,17,20,and 11 compounds with antioxidant activity were identified from roots,stems,leaves,and seeds.The results show that there are certain differences in chemical components and antioxidant components of different parts of A.argyi,which provides data support for the resource utilization and further research and development of A.argyi.
Artemisia argyionline screeningantioxidant activityUPLC-ABTS-Q-TOF-MS2,2'-azino-bis(3-ethylbenzothiazo-line-6-sulfonic acid)diammonium salt
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