摘要
为了分析毛叶香茶菜Isodon japonicus根部和茎叶部分的化学成分异同并进行其质量评价,该文利用UPLC-Q-TOF-MS技术,在系统表征其化学成分的基础上,分析鉴定其主要化合物的结构,并利用对照品建立了其同时含量测定的方法.共在线鉴定或推测了毛叶香茶菜中 34 个主要化合物的结构,包括对照品确定的 14 个化合物,并建立了其中 11 个化合物[丹参素、单咖啡酰酒石酸、新西兰牡荆苷、(1S,2S)-globoidnan B、芦丁、(+)-rabdosiin、(-)-rabdosiin、(1S,2S)-rabdosiin、shimobashiric acid C、迷迭香酸、胡麻素]的 UPLC-UV含量测定方法.11 个化合物的分离度良好,稳定性和重复性良好,且线性范围宽(0.10～520.00 μg·mL-1)、线性好(R2>0.999),平均回收率在 94.72%～104.2%.主成分分析(PCA)结果显示根部和茎叶部分差异明显,能良好聚类分离,采用正交偏最小二乘法判别分析(OPLS-DA)模型筛选到 6 个准确鉴定的主要差异化合物,分别为迷迭香酸、shimobashiric acid C、表诺多星、胡麻素、芦丁、(1S,2S)-rabdosiin.综上,该文建立了一种可以区分毛叶香茶菜不同部位的策略和方法,并能用于毛叶香茶菜的质量控制,为毛叶香茶菜的合理利用和可持续发展提供了依据.
Abstract
In order to analyze the similarities and differences of chemical compositions between the roots and stems and leaves of Isodon japonicus(IJ),this study utilized UPLC-Q-TOF-MS technology to systematically characterize its chemical compositions,ana-lyzed and identified the structure of its main compounds,and established a method for simultaneous determination of its content by refe-rence substance.A total of 34 major compounds in IJ,including 14 reference compounds,were identified or predicted online.Moreo-ver,an UPLC-UV content determination method was developed for 11 compounds[danshensu,caffeic acid,vicenin-2,(1S,2S)-globoidnan B,rutin,(+)-rabdosiin,(-)-rabdosiin,(1S,2S)-rabdosiin,shimobashiric acid C,rosmarinic acid,and pedalitin].The method exhibited excellent separation,stability,and repeatability,with a wide linear range(0.10-520.00 μg·mL-1)and high lineari-ty(R2>0.999).The average recovery rates ranged from 94.72%to 104.2%.The principal component analysis(PCA)demonstrated a clear difference between the roots and stems and leaves of IJ,indicating good separation by cluster.Furthermore,the orthogonal par-tial least squares discriminant analysis(OPLS-DA)model was employed,and six main differentially identified compounds were identi-fied:rosmarinic acid,shimobashiric acid C,epinodosin,pedalitin,rutin,and(1S,2S)-rabdosiin.In summary,this study established a strategy and method for distinguishing different parts of IJ,providing a valuable tool for quality control of IJ and a basis for the ratio-nal utilization and sustainable development of IJ.
基金项目
中央本级重大增减支项目(2060302-2205-03)
浙江省"尖兵领雁"研发攻关计划(2022C03131)
中国中医科学院科技创新工程项目(CI2021A05206)
中国中医科学院科技创新工程项目(CI2023C071YLL)
中国中医科学院科技创新工程项目(CI2021A04504)
中国中医科学院科技创新工程项目(CI2021A04007)
中国中医科学院科技创新工程项目(CI2023E002)
NETL
NSTL
万方数据