首页|基于代谢组学及转录组学研究灰毡毛忍冬不同品种黄酮类成分差异积累的转录调控机制

基于代谢组学及转录组学研究灰毡毛忍冬不同品种黄酮类成分差异积累的转录调控机制

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
为探究湘蕾型和野生型灰毡毛忍冬黄酮类成分差异积累的分子调控机制，该研究以灰毡毛忍冬 2 个品种花、茎和叶为材料，采用超高效液相色谱-质谱联用(UPLC-MS)技术和高通量转录组测序(RNA-seq)技术进行代谢组学和转录组学联合分析，筛选黄酮类差异代谢物、关键差异表达基因及相应转录因子，并随机挑选其中 14 条差异表达基因进行qRT-PCR验证。结果表明，代谢组学分析共筛选出黄酮类化合物生物合成相关途径差异代谢物 17 个，包括柚皮苷查尔酮、芹菜素、槲皮苷等;转录组学分析共筛选出黄酮类化合物生物合成相关途径差异表达基因19条，包括2条CHS、3条CHI等;调控网络分析和加权基因共表达网络分析(WGCNA)筛选出调节黄酮类成分积累的关键酶基因CHS1、FLS1和HCT;同时发现MYB12和LBD4 这2个转录因子可能通过调控关键酶基因CHS1、FLS1和HCT的表达来调控黄酮类成分的生物合成;qRT-PCR结果显示，随机选取的 14 个差异表达基因的表达模式与RNA-seq结果基本一致。该研究初步解析了2个品种灰毡毛忍冬黄酮类成分差异积累的转录调控机制，为进一步阐明其中关键酶基因及相应转录因子对灰毡毛忍冬黄酮类成分积累的调控作用奠定基础。
Transcriptional regulation mechanism of differential accumulation of flavonoids in different varieties of Lonicera macranthoides based on metabonomics and transcriptomics
This study aims to explore the molecular regulatory mechanism of the differential accumulation of flavonoids between'Xianglei'and the wild type of Lonicera macranthoides.The flowers,stems,and leaves of the two varieties of L.macranthoides were collected.Ultra-performance liquid chromatography-mass spectrometry(UPLC-MS)and high-throughput sequencing(RNA-seq)were employed to screen out the differential flavonoids,key differentially expressed genes(DEGs)and transcription factors(TFs).Four-teen DEGs were randomly selected for verification by qRT-PCR.The results showed that a total of 17 differential flavonoids were ob-tained,including naringin chalcone,apigenin,and quercetin.The transcriptomic analysis predicted 19 DEGs associated with fla-vonoids,including 2 genes encoding chitin synthase(CHS)and 3 genes encoding chalcone isomerase(CHI).The regulatory network analysis and weighted gene co-expression network analysis(WGCNA)screen out the key enzyme genes CHS1,FLS1,and HCT regula-ting the accumulation of flavonoids.MYB12 and LBD4 may be involved in the biosynthesis of flavonoids by regulating the expression of key enzyme genes CHS1,FLS1,and HCT.The qRT-PCR and RNA-seq results were similar regarding the expression patterns of the 14 randomly selected DEGs.This study preliminarily analyzed the transcriptional regulatory mechanism for the differential accumulation of flavonoids in the two varieties of L.macranthoides and laid a foundation for further elucidating the regulatory effects of key enzyme genes and TFs on the accumulation of flavonoids.

Lonicera macranthoidesflavonoidsintegrative metabolome and transcriptome analysistranscription factorsregulatory mechanisms

张惊宇、龙雨青、曾娟、付学森、何佳蔚、周日宝、刘湘丹

展开 >

湖南中医药大学 药学院,湖南 长沙 410208

湘产大宗道地药材种质资源及规范化种植重点研究室,湖南 长沙 410208

湖南省普通高等学校中药现代化研究重点实验室,湖南 长沙 410208

湖南省中药饮片标准化及功能工程技术研究中心,湖南 长沙 410208

展开 >

灰毡毛忍冬 黄酮类化合物 转录代谢组学联合分析 转录因子 调控机制

国家自然科学基金湖南省自然科学基金湖南省自然科学基金现代农业产业技术体系建设项目湖南省大学生创新创业训练计划湖南中医药大学研究生创新训练项目湖南省一流专业建设点中药资源与开发项目(2020)湖南中医药大学重点学科中药学科建设项目

823739922021JJ304972021JJ30515CARS-21湘教通2022[174]号-29132023CX152校行发规字[2023]2号

2024

10.19540/j.cnki.cjcmm.20240211.101

中国中药杂志
中国药学会

中国中药杂志

CSTPCD北大核心
影响因子：1.718
ISSN：1001-5302
年,卷(期)：2024.49(10)
  • 34