Preparation and characterization of co-loaded indocyanine green and elemene nano-emulsion in situ gel and its effect on proliferation of breast cancer MCF-7 cells
This study aims to prepare co-loaded indocyanine green(ICG)and elemene(ELE)nano-emulsion(NE)in situ gel(ICG-ELE-NE-gel)and evaluate its physicochemical properties and antitumor activity in vitro.ICG-ELE-NE-gel was prepared by aqueous phase titration and cold solution methods,followed by characterization of the morphology,particle size,corrosion,and photo-thermal conversion characteristics.The human breast cancer MCF-7 cells were taken as the model,combined with 808 nm laser irradia-tion.Cell inhibition rate test and cell uptake test were performed.ICG-ELE-NE was spherical and uniform in size.The average particle size and Zeta potential were(85.61±0.35)nm and(-21.4±0.6)mV,respectively.The encapsulation efficiency and drug loading rate were 98.51%±0.39%and 10.96%±0.24%,respectively.ICG-ELE-NE-gel had a good photothermal conversion effect and good photothermal stability.The dissolution of ICG-ELE-NE-gel had both temperature and pH-responsive characteristics.Compared with free ELE,ICG-ELE-NE-gel combined with near-infrared light irradiation significantly enhanced the inhibitory effect on MCF-7 cells and could be uptaken in large amounts by MCF-7 cells.ICG-ELE-NE-gel was successfully prepared,and its antitumor activity was en-hanced after 808 nm laser irradiation.
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