共载吲哚菁绿与榄香烯纳米乳原位凝胶的制备、表征及其对乳腺癌MCF-7细胞增殖的影响
Preparation and characterization of co-loaded indocyanine green and elemene nano-emulsion in situ gel and its effect on proliferation of breast cancer MCF-7 cells
赵云龙 1赵梦洁 1王华华 1王子昂 1李一菁 1直炜炜 1关延彬 1吴灿 1祝侠丽1
作者信息
- 1. 河南中医药大学 药学院,河南 郑州 450046
- 折叠
摘要
制备共载吲哚菁绿(indocyanine green,ICG)与榄香烯(elemene,ELE)的纳米乳(nano-emulsion,NE)原位凝胶(ICG-ELE-NE-gel),并研究其理化性质和体外抗肿瘤活性.通过水相滴定法和冷溶法制备ICG-ELE-NE-gel,对其形态、粒径、溶蚀、光热转换特性等进行研究;以人乳腺癌MCF-7细胞为模型,并结合 808 nm激光照射,进行细胞抑制率实验与细胞摄取实验.ICG-ELE-NE为类球形,大小均匀,平均粒径和Zeta电位分别为(85.61±0.35)nm和(-21.4±0.6)mV,包封率和载药量分别为98.51%±0.39%和 10.96%±0.24%.ICG-ELE-NE-gel具有良好的光热转换效应,光热稳定性良好,并具有温度和pH双重响应性的溶蚀特性.与游离ELE相比,ICG-ELE-NE-gel结合近红外光照射后对MCF-7 细胞的抑制作用显著增强,且能被MCF-7细胞大量摄取.该研究成功制备了ICG-ELE-NE-gel,该制剂结合 808 nm激光照射后,抗肿瘤活性增强.
Abstract
This study aims to prepare co-loaded indocyanine green(ICG)and elemene(ELE)nano-emulsion(NE)in situ gel(ICG-ELE-NE-gel)and evaluate its physicochemical properties and antitumor activity in vitro.ICG-ELE-NE-gel was prepared by aqueous phase titration and cold solution methods,followed by characterization of the morphology,particle size,corrosion,and photo-thermal conversion characteristics.The human breast cancer MCF-7 cells were taken as the model,combined with 808 nm laser irradia-tion.Cell inhibition rate test and cell uptake test were performed.ICG-ELE-NE was spherical and uniform in size.The average particle size and Zeta potential were(85.61±0.35)nm and(-21.4±0.6)mV,respectively.The encapsulation efficiency and drug loading rate were 98.51%±0.39%and 10.96%±0.24%,respectively.ICG-ELE-NE-gel had a good photothermal conversion effect and good photothermal stability.The dissolution of ICG-ELE-NE-gel had both temperature and pH-responsive characteristics.Compared with free ELE,ICG-ELE-NE-gel combined with near-infrared light irradiation significantly enhanced the inhibitory effect on MCF-7 cells and could be uptaken in large amounts by MCF-7 cells.ICG-ELE-NE-gel was successfully prepared,and its antitumor activity was en-hanced after 808 nm laser irradiation.
关键词
榄香烯/吲哚菁绿/纳米乳/原位凝胶/乳腺癌/光热治疗Key words
elemene/indocyanine green/nanoemulsion/in situ gel/breast cancer/photothermal therapy引用本文复制引用
基金项目
国家自然科学基金(81803740)
河南省高等学校重点科研项目(23A360007)
出版年
2024
NETL
NSTL
万方数据