Active secondary metabolites from an endophytic fungus Fusarium solani MBM-5 of Datura arborea
This study investigated the chemical and biological activity of the secondary metabolites from an endophytic fungus Fusa-rium solani MBM-5 of Datura arborea.A total of six alkenoic acid compounds,including a new compound and five known ones,were isolated from the ethyl acetate extract of F.solani MBM-5 by using the chromatographic methods(open ODS column chromatography,silica gel column chromatography,Sephadex LH-20,and semi-preparative HPLC).The structures of the compounds were identified by using their physical and chemical data,spectroscopic methods(UV,IR,NMR,and HR-ESI-MS),and Mosher's reaction,which were fusaridioic acid E(1),fusaridioic acid C(2),fusaridioic acid A(3),L660282(4),hymeglusin(5),and hymeglnone(6).Compound 1 is new.MTT assay and Griss method were used to evaluate the growth inhibition of all the compounds against two tumor cells,as well as their influence and anti-inflammatory action on the release of NO from LPS-induced RAW264.7 cells.The results showed that compound 5 had strong growth inhibition activity against A549 and HepG2 cell lines,with IC50 values of 4.70 and 13.57 μmol·L-1,respectively.Compounds 1 and 6 significantly inhibited the release of NO from LPS-induced RAW264.7 cells,with IC50 values of 77.00 and 70.33 μmol·L-1,respectively.
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