The chemical constituents from Leucas ciliata belonging to Leucas genus in Lamiaceae were systematically explored by silica gel column chromatography,ODS column chromatography,Sephadex LH-20 gel column chromatography,and preparative high performance liquid chromatography,and seventeen phenolic acids were isolated.The chemical structures of the compounds were identified by their physicochemical properties,spectroscopic data,and literature.They were 4-hydroxyphenethyl ethyl succinate(1),4-hydroxyphenethyl methyl succinate(2),2-(4-hydroxyphenyl)ethyl acetate(3),p-hydroxyphenylethyl anisate(4),cassia cis-trans diphenylpropanoid(5),p-coumaric acid(6),3,4-dihydroxybenzenepropionic acid methyl ester(7),caffeic acid(8),trans-p-hydroxyl ethyl cinnamate(9),methyl p-hydroxybenzeneacetate(10),4-hydroxyphenethyl alcohol(11),syringic acid(12),vanillin(13),protocatechuic acid(14),salicylic acid(15),p-hydroxybenzaldehyde(16),and diorcinol(17).Among them,compound 1 was new,and compounds 2-10,12,14,and 16-17 were isolated from the plants belonging to Leucas genus for the first time.All compounds were obtained from L.ciliata for the first time.The anti-inflammatory activity of compounds 1-17 on NO production in lipopolysaccharide(LPS)-induced mouse leukemia cells of monocyte macrophage(RAW264.7)cells was evaluated.The results showed that compounds 5,7,and 9 exhibited significant anti-inflammatory activity,with IC50 values of(10.14±0.36)-(21.17±0.11)μmol·L-1.
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