Chemical constituents from Clitocybe clavipes and their antitumor activities
This paper aimed to study the chemical constituents from Clitocybe clavipes.Silica gel,ODS,Sephadex LH-20,and semi-preparative HPLC were employed to separate the ethanol extract of C.clavipes.Six compounds were identified by 1 H-NMR,13 C-NMR,and ESI-MS as clavilactone L(1),clavilactone A(2),clavilactone B(3),clavilactone E(4),clavilactone H(5),and clavilactone I(6).Among them,compound 1 was a new meroterpenoid with a 10-membered carbocycle connected to a hydroquinone.The antitumor activities of compounds 1-6 were determined by the methyl thiazolyl tetrazolium(MTT)assay.The results showed that compounds 1-6 exerted inhibitory effects on the proliferation of human gastric cancer cells(MGC-803),human non-small cell lung cancer cells(A549),and cervical cancer cells(HeLa).Compound 1 exhibited significant inhibitory activity against MGC-803 cells,with the half maximal inhibitory concentration(IC50)of 11.76 μmol·L-1.
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