Simultaneous determination of maltol and seventeen saponins in red and black ginseng by UPLC-PDA wavelength switching method
This study developed a UPLC-PDA wavelength switching method to simultaneously determine the content of maltol and seventeen saponins in red and black ginseng and compared the quality differences of two different processed products of red and black ginseng.A Waters HSS T3 column(2.1 mm×100 mm,1.8 μm)at 30℃was adopted,with the mobile phase of acetonitrile(A)and water containing 0.1% phosphoric acid(B)under gradient elution,the flow rate of 0.3 mL·min-1,and the injection volume of 2 μL.The wavelength switching was set at 273 nm within 0-11 min and 203 nm within 11-60 min.The content results of multiple batches of red and black ginseng samples were analyzed by the hierarchical cluster analysis(HCA)and principal component analysis(PCA)to evaluate the quality difference.The results showed that the 18 constituents exhibited good linear relationships within certain concentration ranges,with the correlation coefficients(r)greater than 0.999 1.The relative standard deviations(RSDs)of precision,repeatability,and stability were all less than 5.0% .The average recoveries ranged from 95.93% to 104.2%,with an RSD of 1.8%-4.2% .The content determination results showed that the quality of red and black ginseng samples was different,and the two types of processed products were intuitively distinguished by HCA and PCA.The method is accurate,reliable,and reproducible.It can be used to determine the content of maltol and seventeen saponins in red and black ginseng and provide basic information for the quality evaluation and comprehensive utilization of red and black ginseng.
red ginsengblack ginsengUPLC-PDAwavelength switching methodmaltolsaponins
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