Effect and mechanism of EtOAc extract of Draconis Sanguis on improving atherosclerosis in ApoE-/-mice
This study aims to investigate the effect and mechanism of the EtOAc extract of Draconis Sanguis(DSE)on improving atherosclerosis in ApoE gene knockout(ApoE-/-)mice.The ApoE-/-mice were randomly divided into five groups:control group,model group,positive group treated with ezetimibe of 5 mg·kg-1(EG),and low(100 mg·kg-1)and high dose(200 mg·kg-1)groups of DSE.Except for the control group,all other groups were fed a high-fat diet and administered drugs for 16 successive weeks.After 16 weeks of administration,the body weight,liver,and epididymal fat mass of the mice were measured;the level of blood lipid and the plaque area of the aortic outflow tract were detected to evaluate the efficacy of DSE in vivo.In addition,in vitro cultures of human umbilical vein endothelial cell(HUVEC)were conducted.Oxidative stress of endothelial cells was induced by oxidized low-density lipoprotein(ox-LDL),and the effects of DSE on oxidative stress-related proteins in endothelial cells were examined.The results showed that both doses of DSE significantly improved the epididymal fat mass and index of ApoE-/-mice with atherosclerosis,lowered the levels of plasma total cholesterol,triglyceride,and non-high density lipoprotein cholesterol,and reduced the plaque area of the aortic outflow tract.In vitro experiments confirmed that ox-LDL significantly increased the level of lipid peroxidation marker 4-HNE in HUVEC cells,confirming that DSE improved the degree of atherosclerotic lesions in ApoE-/-mice by inhibiting ox-LDL-induced oxidative stress in vascular endothelial cells.
EtOAc extract of Draconis Sanguisatherosclerosisoxidative stresslipid peroxidation
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