Exploration of cancer biological characteristics in people with different five movements and six climates constitution based on pan-cancer Bulk RNA-Seq
According to the theory of five movements and six climates,the innate constitution plays a crucial role in determining the underlying pathological mechanisms of diseases later in life.Previous studies have demonstrated a close association between the constitution,as defined by the theory of five movements and six climates,and the development of various types of tumors.Furthermore,the tumor subtype determined by the constitution has prognostic implications.This highlights the potential of utilizing the five movements and six climates theory to guide the implementation of precision medicine strategies in the field of oncology.However,no research has yet been conducted to investigate the use of this theory in guiding the development of tumor molecular classification and precision medicine strategies.The objective of this research is to uncover the biological characteristics of each constitution within a pan-cancer cohort and identify potential anti-tumor drugs that are applicable to patients with different constitutional types.By doing so,we aim to contribute to the establishment of a precision medicine strategy for tumors derived from the original concepts of traditional Chinese medicine(TCM).In this study,we obtained pan-cancer Bulk RNA-Seq data from UCSC Xena,GWAS cohort data from the UK Biobank,and cis-eQTLs data from eQTLGen and GTEx V8.We employed machine learning methods to screen for hub genes associated with each constitution.Subsequently,we utilized bioinformatics tools to explore the biological characteristics of each constitution defined by the theory of five movements and six climates.Further,potential anti-tumor drugs suitable for patients with different constitutional types were identified through mendelian randomization,molecular docking,and drug-like prediction techniques.Within the pan-cancer cohort,significant differences were observed among different constitutions in terms of progression-free interval,biological functions,immune cell abundance,tumor drug sensitivity,and immunotherapy response.These findings suggest that the five movements and six climates theory can guide tumor molecular classification and the development of precision medicine strategies.Moreover,the biological characteristics inherent to each constitution partially shed light on the scientific implications of Chinese medicine theories,offering a fresh perspective towards clinical cancer treatment.Through molecular docking and drug-like prediction,several potential anti-tumor drugs such as 17-beta-estradiol,serotonin,trans-resveratrol,and linoleic acid were identified.Overall,the utilization of multi-omics approaches provides a powerful tool to unravel the scientific foundations of TCM theories.The elucidation of the multi-omics features associated with each constitution in tumors serves as the basis for applying the five movements and six climates theory to tumor molecular classification and the development of precision medicine strategies.
pan-cancerfive movements and six climatestumour molecular classificationintegrated Chinese and western medi-cinedrug virtual screening
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NSTL
万方数据
