首页|土壤相对含水量对蜜环菌生长特性及乌天麻种苗生产的影响

土壤相对含水量对蜜环菌生长特性及乌天麻种苗生产的影响

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天麻种苗的存活率、产量、单重与土壤相对含水量(RWC)及天麻共生蜜环菌的生长特性密不可分，研究土壤RWC对蜜环菌菌株生长特性及乌天麻种苗的影响，可为乌天麻良种繁育及乌天麻共生蜜环菌优良菌株筛选提供指导。该研究根据30 株蜜环菌菌株在活化培养基上的生长特性将其分为 4 类，再从每类蜜环菌菌株中筛选 2 株生长特性指标优的菌株与乌天麻米麻及阔叶树修剪枝进行箱式控水熟料栽培试验。结果发现具有根状菌索单轴分枝的蜜环菌菌株是与乌天麻共生的优良菌株，如第Ⅲ类、第Ⅳ类蜜环菌;土壤RWC对不同类型蜜环菌的生长特性指标，乌天麻种苗的存活率、产量、单重有显著影响;土壤RWC为 75%时，蜜环菌菌株生长速度、直径、分支数、生物量及乌天麻种苗存活率、产量、单重均最优，通过人为控制土壤的RWC，在大棚内将乌天麻米麻与优良蜜环菌菌株及阔叶树修剪枝伴栽可以获得优良的乌天麻种苗，为乌天麻的高效生产、米麻利用提供参考。
Effects of soil relative water content on growth characteristics of Armillaria spp.and seedling production of Gastrodia elata f.glauca
The seedling survival rate,yield,and individual weight of Gastrodia elata is closely related to the soil relative water con-tent(RWC)and the growth characteristics of the associated fungi Armillaria spp.This study explored the effects of the soil RWC on the growth characteristics of Armillaria spp.and the seedling production of G.elata f.glauca,aiming to provide guidance for breeding G.elata f.glauca and selecting elite strains of Armillaria.According to the growth characteristics on the medium for activation,thirty strains of Armillaria were classified into 4 clusters.Two strains with good growth indicators were selected from each cluster and cultiva-ted with immature tuber(Mima)and the branches of the broad-leaved trees in a water-controlled box.The results showed that the Ar-millaria clusters with uniaxial branches of rhizoid cords,such as clusters Ⅲ and Ⅳ,were excellent clusters in symbiosis with G.elata f.glauca.The soil RWC had significant effects on the growth characteristics of Armillaria strains and the seedling survival rate,yield,and individual weight of G.elata f.glauca.The growth characteristics of Armillaria strains and the seedling survival rate,yield,and individual weight of G.elata f.glauca in the case of the soil RWC being 75%were significantly better than those in the case of other soil RWC.Cultivating Mima with elite strains of Armillaria,together with branches of broad-leaved trees,in the greenhouses with the artificial control of the soil RWC,can achieve efficient seedling production and Mima utilization of G.elata f.glauca.

relative water content(RWC)of soilseedling of Gastrodia elata f.glaucaArmillaria straingrowth indicatoryieldindividual weightridge regression

王丽、张金渝、袁媛、刘天睿

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云南省农业科学院 药用植物研究所,云南 昆明 650000

中国中医科学院 中药资源中心道地药材品质保障与资源持续利用全国重点实验室,北京 100700

中国中医科学院 中医药健康产业研究所,江西 南昌 330115

昭通学院 云南省天麻与真菌共生生物学重点实验室,云南 昭通 657000

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土壤相对含水量(RWC) 乌天麻种苗 蜜环菌菌株 生长特性指标 产量 单重 岭回归

国家自然科学基金中国中医科学院科技创新工程重大攻关项目中央级公益性科研院所基本科研业务费专项中央级公益性科研院所基本科研业务费专项云南省天麻与真菌共生生物学重点实验室开放基金云南省科技人才与平台计划

31960391CI2021B014ZZXT202217ZZ16-ND-12TMKF2023B01202205AD160025

2024

10.19540/j.cnki.cjcmm.20240215.101

中国中药杂志
中国药学会

中国中药杂志

CSTPCD北大核心
影响因子：1.718
ISSN：1001-5302
年,卷(期)：2024.49(12)
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