Authenticity discrimination for Galli Gigerii Endothelium Corneum based on UPLC-MS/MS and peptidomics
Peptidomics was employed to systematically analyze the characteristic peptides in Galli Gigerii Endothelium Corneum and its adulterants and establish a method for distinguishing Galli Gigerii Endothelium Corneum from its adulterants,including the gizzard membranes from ducks,geese,and pigeons.UPLC-Q-Exactive Orbitrap-MS was combined with multivariate statistical analysis to ana-lyze the peptides in Galli Gigerii Endothelium Corneum and its adulterants.The structures of peptides were identified by pNovo com-bined with manual recognition of spectra,and synthetic peptide standards were used for validation.LC-MS/MS was used to optimize the sample pre-processing conditions,including the extraction procedure,extraction time,extraction solvents,and solvent volumes,for the characteristic peptide LESY in Galli Gigerii Endothelium Corneum.Multiple reaction monitoring(MRM)in the ESI+mode with m/z 511.24→269.11 and 511.24→243.13 as detection ions was employed for qualitative and quantitative analyses.The established UPLC-MS/MS method demonstrated good specificity,stability,and durability.The content of LESY in 16 batches of Galli Gigerii En-dothelium Corneum samples ranged from 55.03 to 113.36 μg·g-1.Additionally,a qualitative detection method for the common peptide RDPVLVSR in adulterants was established with m/z 471.28→785.45 and 471.28→670.41 as the detection ions.This study estab-lished a convenient,rapid,and accurate detection method for the characteristic peptides in Galli Gigerii Endothelium Corneum and its adulterants.The method possesses good specificity,stability,and durability,providing a valuable reference for the identification and quality control of Galli Gigerii Endothelium Corneum and other traditional Chinese medicines derived from animal sources.
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