桂枝水煎液的化学成分研究
Chemical constituents from Cinnamomi Ramulus decoction
刘青 1刘珍伶 2易立涛1
作者信息
- 1. 华侨大学 化工学院 制药工程系,福建 厦门 361021
- 2. 兰州大学 功能有机分子化学国家重点实验室,甘肃 兰州 730000
- 折叠
摘要
采用RP-18 反相柱色谱、硅胶柱色谱、Sephadex LH-20 柱色谱以及半制备型高效液相色谱等手段对桂枝Cinnamomi Ramulus水煎液乙酸乙酯萃取物进行化学成分研究,从中分离得到 6 个化合物,通过现代波谱学分析,分别鉴定为 4,5,10,11-tetrahydroxybisabol-7-ene(1)、4,5,10,11-tetrahydroxybisabolin(2)、1-苯基-1,2,3-丙三醇(3)、(+)-lyoniresinol(4)、苯甲酸(5)和decumbic acid(6).其中化合物 1 为 1 个新的没药烷型倍半萜,化合物 2、3 为首次从桂枝中分离得到.对没药烷型倍半萜化合物 2 进行抗炎活性和人胰腺癌细胞(PANC-1细胞)的细胞毒活性测试,结果显示化合物 2 在 40 μmol·L-1 浓度时对NO的抑制率为 32.9%;在 20 μmol·L-1 时对PANC-1细胞的生长抑制率为 14.5%,未显示出明显活性.
Abstract
Six compounds were isolated from the ethyl acetate extracts of Cinnamomi Ramulus decoction by RP-18,silica gel,Sephadex LH-20 column chromatography,together with prep-HPLC methods.Based on HR-ESI-MS,MS,1D and 2D NMR spectral analyses,the structures of the six compounds were identified as 4,5,10,11-tetrahydroxybisabol-7-ene(1),4,5,10,11-tetrahydroxybisabolin(2),1-phenyl-1,2,3-glycerol(3),(+)-lyoniresinol(4),benzoic acid(5),and decumbic acid(6).Compound 1 was a new bisabolene-type sesquiterpene,and compounds 2 and 3 were isolated from the Cinnamomi Ramulus for the first time.Moreover,the bisabolene-type sesquiterpene(2)was assayed for its anti-inflammatory activity and cytotoxicity to human pancreatic cancer cells(PANC-1 cells).Results showed that compound 2 exhibited an inhibitory rate of 32.9%on nitric oxide(NO)at a dose of 40 μmol·L-1 and a proliferation inhibition rate of 14.5%against PANC-1 cells at a dose of 20 μmol·L-1.It did not demonstrate significant activity.
关键词
桂枝水煎液/化学成分/没药烷型倍半萜/抗炎活性/PANC-1细胞毒活性Key words
Cinnamomi Ramulus decoction/chemical constituents/bisabolene-type sesquiterpene/anti-inflammatory/cytotoxicity to PANC-1 cells引用本文复制引用
基金项目
福建省自然科学基金(2022J01315)
出版年
2024
NETL
NSTL
万方数据