Chemical constituents from Cinnamomi Ramulus decoction
Six compounds were isolated from the ethyl acetate extracts of Cinnamomi Ramulus decoction by RP-18,silica gel,Sephadex LH-20 column chromatography,together with prep-HPLC methods.Based on HR-ESI-MS,MS,1D and 2D NMR spectral analyses,the structures of the six compounds were identified as 4,5,10,11-tetrahydroxybisabol-7-ene(1),4,5,10,11-tetrahydroxybisabolin(2),1-phenyl-1,2,3-glycerol(3),(+)-lyoniresinol(4),benzoic acid(5),and decumbic acid(6).Compound 1 was a new bisabolene-type sesquiterpene,and compounds 2 and 3 were isolated from the Cinnamomi Ramulus for the first time.Moreover,the bisabolene-type sesquiterpene(2)was assayed for its anti-inflammatory activity and cytotoxicity to human pancreatic cancer cells(PANC-1 cells).Results showed that compound 2 exhibited an inhibitory rate of 32.9%on nitric oxide(NO)at a dose of 40 μmol·L-1 and a proliferation inhibition rate of 14.5%against PANC-1 cells at a dose of 20 μmol·L-1.It did not demonstrate significant activity.
Cinnamomi Ramulus decoctionchemical constituentsbisabolene-type sesquiterpeneanti-inflammatorycytotoxicity to PANC-1 cells
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