基于电感耦合等离子体质谱分析金银花和山银花多元素差异的质量控制模式研究

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中文摘要：大量研究表明金银花和山银花在有机物上的确具有显著性差异，但仍然缺少有关两者间无机元素方面的研究。该研究通过电感耦合等离子体质谱(inductively coupled plasma mass spectrometry，ICP-MS)建立非靶向元素组方法，比较金银花和山银花在无机元素上的整体差异，并筛选其差异标志物。同时通过靶向方法定量分析这些差异标志物。非靶向元素组结果表明，得到的数学模型能够较好地反映金银花和山银花在元素含量的差异。以变量重要性投影值(VIP)>2。0，P<0。01 和倍数变化值(FC)>1。2 或<0。80 为筛选原则，确认了锰(55 Mn)、铋(209 Bi)、镉(111 Cd)、铷(85 Rb)等 4 种无机元素为两者的差异标志物。靶向定量结果表明，金银花中209 Bi的含量显著高于山银花，而山银花中55 Mn、111 Cd和85 Rb显著高于金银花。该研究所建立的基于ICP-MS的非靶向和靶向元素组方法可显著反映金银花和山银花的无机元素的整体差异;从元素的角度探讨两者间差异，可部分反映两者药性上的差异，也为进一步研究金银花与山银花中无机元素质量控制模式的建立和其药理功效奠定了基础。

外文标题：Quality control mode between Lonicera macranthoides and L.japonica based on multi-elemental difference through inductively coupled plasma mass spectrometry

外文摘要：Numerous studies show that Lonicera macranthoides and L.japonica have significant differences in organic matter.However,there is still a lack of research on inorganic elements between them.In this study,a non-targeted elemental metabolomics method was established by inductively coupled plasma mass spectrometry(ICP-MS),so as to compare the overall differences of inorganic elements between L.macranthoides and L.japonica.In addition,the differential markers were screened,and these differential markers were quantitatively analyzed by the targeted method.The non-targeted elemental metabolomics showed that the established mathematical model could reflect the difference in element content between L.macranthoides and L.japonica.Four inorganic elements such as 55 Mn,209 Bi,111 Cd,and 85 Rb were confirmed as the differential markers of L.macranthoides and L.japonica based on the screening principles of variable importance in the projection(VIP)value>2.0,P<0.01 and fold change(FC)value>1.2 or<0.80.The targeted quantitative results showed that the content of 209 Bi in L.japonica was significantly higher than that in L.macranthoides,while 55 Mn,111 Cd,and 85 Rb in L.macranthoides were significantly higher than that in L.japonica.The non-targeted and targeted elemental metabolomics methods based on ICP-MS can significantly reflect the overall differences in inorganic elements between L.macranthoides and L.japonica.Exploring the differences between them from the perspective of elements can partly reflect the differences in their drug properties and lay a foundation for further study on the quality control mode of inorganic elements in L.macranthoides and L.japonica and their pharmacological effects.

外文关键词：

inductively coupled plasma mass spectrometryLonicera macranthoideLonicera japonicatargetednon-targeted

作者：

刘恃君、汤杨黔南、陆玉爱、侯师艺、戈玉莹、严婧、李亚梅、夏伯候

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作者单位：

湖南中医药大学药学院湘产大宗药材品质评价湖南省重点实验室,湖南长沙 410208

关键词：

电感耦合等离子体质谱金银花山银花靶向非靶向

基金：

湖南省科技创新项目湖南省自然科学基金湖南省教育厅重点项目湖南省卫生健康委项目湖南中医药大学重点学科中药学科建设项目

项目编号：

2021RC40642022JJ5000823A0307B202313057639校行发规字[2023]2号

出版年：

2024

DOI：

10.19540/j.cnki.cjcmm.20240314.301

中国中药杂志

中国药学会

中国中药杂志

CSTPCD北大核心

影响因子：1.718

ISSN：1001-5302

年,卷(期)：2024.49(12)

参考文献量23