首页|人参皂苷Rg1生物合成相关候选基因的鉴定和功能初步研究

人参皂苷Rg1生物合成相关候选基因的鉴定和功能初步研究

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
人参Panax ginseng为五加科人参属多年生草本植物，其主要活性成分为人参皂苷。在已报道的单体皂苷中，人参皂苷Rg1不仅药用功能广泛、含量丰富，且是《中国药典》中用于鉴定人参品质的主要单体皂苷种类之一。人参皂苷Rg1的生物合成主要催化途径目前已较为明晰，这为解析人参皂苷Rg1的生物合成与调控机制奠定了基础。但是人参皂苷Rg1的合成涉及其他复杂过程，可能还有许多调控基因及催化酶基因，需要进行更加深入、全面的研究。因此，该研究基于前期所得的344份四年生人参根样本的转录组数据库及其对应的人参皂苷Rg1含量表型，利用R语言中的DEseq2分析获得了随人参皂苷Rg，含量变化的217条显著差异表达基因，并在此基础上通过WGCNA获得其中的40条hub基因，进一步利用Pearson相关分析得到其中20条与人参皂苷Rg1含量显著相关的候选基因群，以上基因可注释到初级代谢、次生代谢等多个代谢过程中。随后通过在MeJA诱导的人参不定根中的表达分析，初步验证了其中16条基因响应MeJA诱导对人参皂苷Rg1生物合成的影响。最后从16条基因中随机抽取1条，通过遗传转化和荧光定量的方法进一步验证基因的功能，并确认该研究方法的合理性。以上所得结果将为人参皂苷Rg1的合成调控机制深入研究奠定基础，并为人参皂苷Rg1的工厂化生产提供基因资源。
Identification and functional characterization of candidate genes involved in biosynthesis of ginsenoside Rg1
Panax ginseng is a perennial herb with the main active compounds of ginsenosides.Among the reported ginsenosides,ginsenoside Rg1 not only has a wide range of medicinal functions and abundant content but also is one of the major ginsenoside for the quality evaluation of this herb in the Chinese Pharmacopoeia.The main biosynthesis pathway of ginsenoside Rg1 in P.ginseng has been clarified,which lays a foundation for the comprehensive and in-depth analysis of the biosynthesis and regulatory mechanism of ginseno-side Rg1.However,the biosynthesis of ginsenoside Rg1 is associated with other complex processes involving a variety of regulatory genes and catalyzing enzyme genes,which remain to be studied comprehensively.With the transcriptome data of 344 root samples from 4-year-old P.ginseng plants and their corresponding ginsenoside Rg1 content obtained in the previous study,this study screened out 217 differentially expressed genes(DEGs)with Rg1 content changes by DEseq2 analysis in R language.Furthermore,the weighted gene co-expression network analysis(WGCNA)revealed 40 hub genes among the DEGs.Pearsoncorrelation analysis was further per-forned to yield 20 candidate genes significantly correlated with ginsenoside Rg1 content,and these genes were annotated to multiple metabolic processes including primary metabolism and secondary metabolism.Finally,the treatment of P.ginseng adventitious roots with methyl jasmonate indicated that 16 of these genes promoted the biosynthesis of ginsenoside Rg1 in response to methyl jasmonate in-duction.Finally,one of the 16 genes was randomly selected to verify the function of the gene by genetic transformation and qRT-PCR and to confirm the rationality of the methodology of this study.The above results lay a foundation for studying the mechanism for regula-tion on the synthesis of ginsenoside Rg1 and provide genetic resources for the industrial production of ginsenoside Rg1.

candidate functional genesginsenoside Rg1differential analysisweighted gene co-expression network analysis(WGCNA)methyl jasmonate regulationgenetic transformationqRT-PCR

刘芮岑、李俐、王超凡、王义、张美萍

展开 >

吉林农业大学生命科学学院,吉林长春 130118

吉林省人参基因资源开发与利用工程研究中心吉林农业大学,吉林 长春 130118

候选功能基因 人参皂苷Rg1 差异分析 加权基因共表达网络分析(WGCNA) 茉莉酸甲酯调控 遗传转化 荧光定量PCR

吉林省科技厅项目

20210402043GH

2024

10.19540/j.cnki.cjcmm.20240413.101

中国中药杂志
中国药学会

中国中药杂志

CSTPCD北大核心
影响因子：1.718
ISSN：1001-5302
年,卷(期)：2024.49(13)