The chemical constituents from the stems and leaves of Artocarpus tonkinensis in Artocarpus of Moraceae were systemati-cally studied by means of silica gel,octadecylsilyl(ODS),and Sephadex LH-20 gel column chromatographies,as well as preparative high-performance liquid chromatography(Pre-HPLC)and a variety of chromatographic separation techniques.The spectral data and physicochemical properties of the compounds were obtained from separation and compared with those of the compounds reported in the literature.As a result,11 compounds isolated from the 90％ethanol extract of the stems and leaves of A.tonkinensis were identified as artocatonkine(1),5,6,7,4'-tetramethoxyflavone(2),apigenin-4'-O-β-D-glucoside(3),rayalinol(4),psorachalcone A(5),4-ketopinoresinol(6),ficusesquilignan B(7),pinnatifidanin Al(8),pinnatifidanin A(9),O-methylmellein(10),and trans-4-hydroxymellein(11).Among these compounds,compound 1 was a new prenylated flavone,and compounds 2-11 were isolated from the plants belonging to the genus Artocarpus for the first time.Furthermore,all compounds 1-11 were evaluated for their anti-rheuma-toid arthritis activities,and the MTS method was used to measure their inhibitory effects on the proliferation of synovioblasts in vitro.The results of activity evaluation showed that flavonoid compounds 1-3,5,and lignan compounds 8 and 9 displayed significant anti-rheumatoid arthritis activities,showing the IC50 values in inhibiting the proliferation of synovioblasts MH7A from(6.38±0.06)µmol L-1 to(168.58±0.28)µmol·L-1.
Artocarpus tonkinensisflavonoidslignansphenolic acidsinhibitory effects on proliferation of synovioblasts
NETL
NSTL
万方数据
