摘要
综合运用硅胶柱色谱、ODS柱色谱、Sephadex LH-20凝胶柱色谱以及制备型HPLC等多种色谱分离技术对桑科波罗蜜属植物胭脂Artocarpus tonkinensis 枝叶中的化学成分进行系统研究.根据分离得到化合物的波谱数据和理化性质,并通过与文献中已经报道的相应化合物的波谱数据和理化性质进行对比,鉴定了从胭脂枝叶的90％乙醇提取物中分离得到的11个化合物,分别鉴定为 artocatonkine(1)、5,6,7,4'-四甲氧基黄酮(2)、芹菜素-4'-O-β-D-葡萄糖苷(3)、rayalinol(4)、psorachalcone A(5)、4-ketopinoresinol(6)、ficusesquilignan B(7)、pinnatifidanin AI(8)、pinnatifidanin A(9)、O-methylmellein(10)、trans-4-hydroxy-mellein(11).其中化合物1为1个新异戊烯基取代黄酮,化合物2～11为首次从胭脂属植物中分离得到.此外,为了评价化合物1～11的抗类风湿性关节炎活性,采用MTS法对分离得到的化合物1～11的体外抑制滑膜细胞增殖的活性进行了测定.活性评价结果表明黄酮类化合物1～3、5以及木脂素类化合物8、9表现出了较为显著的抗类风湿性关节炎活性,它们对MH7A滑膜成纤维细胞增殖抑制活性的IC50为(6.38±0.06)～(168.58±0.28)μmol·L-1.
Abstract
The chemical constituents from the stems and leaves of Artocarpus tonkinensis in Artocarpus of Moraceae were systemati-cally studied by means of silica gel,octadecylsilyl(ODS),and Sephadex LH-20 gel column chromatographies,as well as preparative high-performance liquid chromatography(Pre-HPLC)and a variety of chromatographic separation techniques.The spectral data and physicochemical properties of the compounds were obtained from separation and compared with those of the compounds reported in the literature.As a result,11 compounds isolated from the 90％ethanol extract of the stems and leaves of A.tonkinensis were identified as artocatonkine(1),5,6,7,4'-tetramethoxyflavone(2),apigenin-4'-O-β-D-glucoside(3),rayalinol(4),psorachalcone A(5),4-ketopinoresinol(6),ficusesquilignan B(7),pinnatifidanin Al(8),pinnatifidanin A(9),O-methylmellein(10),and trans-4-hydroxymellein(11).Among these compounds,compound 1 was a new prenylated flavone,and compounds 2-11 were isolated from the plants belonging to the genus Artocarpus for the first time.Furthermore,all compounds 1-11 were evaluated for their anti-rheuma-toid arthritis activities,and the MTS method was used to measure their inhibitory effects on the proliferation of synovioblasts in vitro.The results of activity evaluation showed that flavonoid compounds 1-3,5,and lignan compounds 8 and 9 displayed significant anti-rheumatoid arthritis activities,showing the IC50 values in inhibiting the proliferation of synovioblasts MH7A from(6.38±0.06)µmol L-1 to(168.58±0.28)µmol·L-1.
基金项目
海南省重点研发计划项目(ZDYF2022SHFZ028)
海南省重点研发计划项目(ZDYF2024SHFZ145)
海南省重点研发计划项目(ZDYF2024XDNY148)
国家自然科学基金项目(32070390)
国家自然科学基金项目(22067005)
国家自然科学基金项目(21967008)
海南省省级大学生创新训练项目(S202311658012)
海南省省级大学生创新训练项目(S202311658011)
海口市科技计划项目创新平台建设项目(2017050)
海口市科技计划项目创新平台建设项目(2020054)
NETL
NSTL
万方数据