Chemical constituents from stems and leaves of Artocarpus tonkinensis and their inhibitory effects on proliferation of synovioblasts in vitro
The chemical constituents from the stems and leaves of Artocarpus tonkinensis in Artocarpus of Moraceae were systemati-cally studied by means of silica gel,octadecylsilyl(ODS),and Sephadex LH-20 gel column chromatographies,as well as preparative high-performance liquid chromatography(Pre-HPLC)and a variety of chromatographic separation techniques.The spectral data and physicochemical properties of the compounds were obtained from separation and compared with those of the compounds reported in the literature.As a result,11 compounds isolated from the 90％ethanol extract of the stems and leaves of A.tonkinensis were identified as artocatonkine(1),5,6,7,4'-tetramethoxyflavone(2),apigenin-4'-O-β-D-glucoside(3),rayalinol(4),psorachalcone A(5),4-ketopinoresinol(6),ficusesquilignan B(7),pinnatifidanin Al(8),pinnatifidanin A(9),O-methylmellein(10),and trans-4-hydroxymellein(11).Among these compounds,compound 1 was a new prenylated flavone,and compounds 2-11 were isolated from the plants belonging to the genus Artocarpus for the first time.Furthermore,all compounds 1-11 were evaluated for their anti-rheuma-toid arthritis activities,and the MTS method was used to measure their inhibitory effects on the proliferation of synovioblasts in vitro.The results of activity evaluation showed that flavonoid compounds 1-3,5,and lignan compounds 8 and 9 displayed significant anti-rheumatoid arthritis activities,showing the IC50 values in inhibiting the proliferation of synovioblasts MH7A from(6.38±0.06)µmol L-1 to(168.58±0.28)µmol·L-1.
Artocarpus tonkinensisflavonoidslignansphenolic acidsinhibitory effects on proliferation of synovioblasts
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