Two new pinophol derivatives from endophytic fungus Penicillium herquei JX4 hosted in Ceriops tagal
An OSMAC strategy was used to study secondary metabolites and anti-inflammatory activities of the endophytic fungus Penicillium herquei JX4 hosted in Ceriops tagal.The PDB ferment of fungus P.herquei JX4 was isolated,purified,and identified by using silica gel column chromatography,gel column chromatography,octadecylsilyl(ODS)column chromatography,and semi-prepara-tive high-performance liquid chromatography.Two new pinophol derivatives,pinophol H(1)and pinophol Ⅰ(2)were isolated and identified,and they were evaluated in terms of the inhibitory activities against the nitric oxide(NO)production induced by lipopolysac-charide(LPS)in mouse macrophage RAW264.7 cells.The results showed that compound 1 had significant inhibitory activity on NO production,with an IC50 value of 8.12 µmol·L-1.
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