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航天诱变葛麻姆SP1代群体遗传变异分析

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为探讨太空环境对葛麻姆Pueraria montana的诱变效应，筛选出生长状态佳、异黄酮成分含量高的优良种质，实验对79株航天葛麻姆和10株对照葛麻姆进行农艺性状观测、花朵异黄酮成分与AFLP荧光分子标记，用Excel 2019、SPSS 25。0、NTSYSpc-2。11F、Popgen 32等软件进行遗传多样性分析并聚类。结果表明，航天组的叶片毛姿态与花结构等2个性状发生变化，数量性状的CV、H'均高于对照组，聚类后有5株航天葛麻姆分出。航天组共有10株葛麻姆花的6"-O-木糖鸢尾苷与鸢尾苷显著高于对照组，二者总量均超过11％，聚类后共有9株航天葛麻姆分出。从64对引物中筛选出9对多态性引物，多态性位点数共1 620个，多态位点百分率(PPL)平均值为83。33％;Nei's基因多样性指数(H)平均值为0。192 2、Shannon's信息指数(I)平均值为0。305 2，聚类后有4株航天植株分出。综合筛选结果，航天组30号、66号、89号葛麻姆受到太空环境的诱变作用较大，主要表现为生长状况更佳、花朵异黄酮含量更高，且30号有花结构变异、花重达到对照组2倍，可作为后续试验的重点研究材料。
Genetic diversity of SP1 population of space mutagenized Pueraria montana
To explore the mutagenic effect of the space environment on Pueraria montana and select the elite germplasm with good growth conditions and high isoflavone content,this study observed the agronomic traits,determined the flower isoflavone content,and labeled amplified fragment length polymorphism(AFLP)fluorescent molecular markers of 79 P.montana plants exposed to space mu-tagenesis(SP1 group)and 10 control plants of P.montana(CK group).Excel 2019,SPSS 25.0,NTSYSpc-2.11F,and Popgen 32 were employed to analyze the genetic diversity and perform the cluster analysis.The results showed that the SP1 group presented changed leaf hairy attitude and flower structure and higher CV and H'of quantitative traits than the CK group.The cluster analysis screened out five plants in the SP1 group.Ten P.montana plants in the SP1 group had higher content of 6"-O-xylosyl-tectoridin and tectoridin in the flowers than the control group,with the total content of both exceeding 11％.After clustering,9 plants in the SP1 group were separated.Nine pairs of polymorphic primers were screened out frrom 64 pairs of primers.A total of 1 620 polymorphic loci were detected,with the average percentage of polymorphic loci(PPL)of 83.33％.The average Nei's gene diversity index(H)and Shannon's information index(I)were 0.192 2 and 0.305 2,respectively.After clustering,4 plants in the SP1 group were screened out.According to the above results,plants No.30,No.66,and No.89 in the SP1 group were subjected to greater mutagenic effect by the space environment and presented better growth and higher flower isoflavone content.Moreover,plant No.30 showed the flower structure variation and flower weight two times of that in the CK group.These plants can be used as key materials for the subsequent ex-periments.

space breedingagronomic traitscontent determinationamplified fragment length polymorphism(AFLP)Pueraria montanagenetic diversity

胡继丹、董丽华、朱卫丰、王斌、陈一鸣、王晓云、朱玉野、葛菲

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江西中医药大学药学院,江西 南昌 330004

航天育种 农艺性状 含量测定 扩增片段长度多态性(AFLP) 葛麻姆 遗传多样性

国家重点研发计划项目子课题江西省农村农业厅项目江西中医药大学创新创业训练计划项目

2017YFC1702901JXARS-16X202310412180

2024

10.19540/j.cnki.cjcmm.20240415.102

中国中药杂志
中国药学会

中国中药杂志

CSTPCD北大核心
影响因子：1.718
ISSN：1001-5302
年,卷(期)：2024.49(14)