Genome-wide screening and bioinformatics analysis of Cannabis sativa LecRLK gene family
Lectin receptor-like kinase(LecRLK)is a class of phytokinase with lectin conserved domain,which plays an important role in plant resistance to biological and abiotic stresses,as well as plant growth and development.Cannabis sativa is an important multi-purpose plant,widely used in food,textile,medicine,and other fields.Genome-wide screening and expression analysis of the LecRLK family of C.sativa were performed in this paper,so as to provide scientific reference for functional analysis of the LecRLK family of C.sativa.Based on BLAST and HMM methods,93 LecRLKs were identified in the whole genome of C.sativa,including 69 G types,23 L types,and one C types.Subsequently,a series of bioinformatics analyses were performed on the LecRLK family members,and the physicochemical properties of the protein of the LecRLK family members were initially revealed.The prediction of cis-acting elements of promoters in family members showed that family members were regulated by hormones and stress response.The expression analysis showed that some family members were highly expressed in the roots,which may participate in the process of stress resistance.Several members were highly expressed in female flowers and may be involved in female flower development.This study provides a theoretical basis for further study of LecRLK gene function.Meanwhile,the expression analysis screens candidate LecRLK members who may participate in the resistance of C.sativa,which provides a theoretical basis for the subsequent selection of C.sativa varieties against resistance.
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