摘要
Trihelix转录因子的功能使其在许多非生物逆境胁迫中都具有重要作用,尤其表现为参与低温、干旱、洪涝、盐碱、脱落酸、茉莉酸甲酯等众多非生物胁迫的信号途径.然而目前对人参Trihelix基因家族的研究还较少.该研究从人参基因组数据库中鉴定筛选出41个Trihelix基因家族成员,使用生物信息学方法分析家族成员理化性质、顺式作用元件、亚细胞定位、染色体定位与非生物胁迫诱导的表达模式.结果表明人参Trihelix家族成员85％定位于细胞核中,Trihelix蛋白二级结构以无规则卷曲和a螺旋为主.在Trihelix的启动子区域鉴定出了与低温、激素响应、生长发育等多种非生物胁迫相关的顺式作用调节元件.通过对模式植物拟南芥和人参进行种间的Trihelix转录因子共线性分析发现人参与拟南芥之间共有共线性基因对19个,仅3、6、12号染色体不存在共线性基因对.qRT-PCR分析结果表明基因GWHGBEIJ010320.1在低温胁迫下表达量显著上调,显著响应低温胁迫.该研究为今后探讨人参Trihelix转录因子在人参非生物胁迫以及人参生长发育过程中的功能奠定了基础.
Abstract
The function of the Trihelix transcription factor is that it plays an important role in many abiotic stresses,especially in the signaling pathway of low temperature,drought,flood,saline,abscisic acid,methyl jasmonate,and other abiotic stresses.However,there are few studies on the Trihelix gene family of ginseng.In this study,41 Trihelix gene family members were identified and screened from the ginseng genome database,and their physicochemical properties,cis-acting elements,subcellular localization,chromosomal assignment,and abiotic stress-induced expression patterns were analyzed by bioinformatics methods.The results showed that 85％of Trihelix family members of ginseng were located in the nucleus,and the main secondary structure of Trihelix protein was random coil and α helix.In the promoter region of Trihelix,cis-acting regulatory elements related to various abiotic stresses such as low temperature,hormone response,and growth and development were identified.Through the collinearity analysis of interspecific Trihelix transcription factors of model plants Arabidopsis thaliana and ginseng,19 collinear gene pairs were found between A.thaliana and ginseng,and no collinear gene pairs existed on chromosomes 3,6,and 12 only.qRT-PCR analysis showed that the expression of GWHGBEIJ010320.1 was significantly up-regulated under low temperature stress,a significant response to low temperature stress.This study lays a foundation for further research on the role of the Trihelix transcription factor of ginseng in abiotic stress,as well as the growth and development of ginseng.
基金项目
国家自然科学基金联合基金项目(U21A20405)
吉林省科技发展计划项目(20220401112YY)
NETL
NSTL
万方数据