Identification and expression pattern analysis of Trihelix transcription factor family genes of ginseng
The function of the Trihelix transcription factor is that it plays an important role in many abiotic stresses,especially in the signaling pathway of low temperature,drought,flood,saline,abscisic acid,methyl jasmonate,and other abiotic stresses.However,there are few studies on the Trihelix gene family of ginseng.In this study,41 Trihelix gene family members were identified and screened from the ginseng genome database,and their physicochemical properties,cis-acting elements,subcellular localization,chromosomal assignment,and abiotic stress-induced expression patterns were analyzed by bioinformatics methods.The results showed that 85％of Trihelix family members of ginseng were located in the nucleus,and the main secondary structure of Trihelix protein was random coil and α helix.In the promoter region of Trihelix,cis-acting regulatory elements related to various abiotic stresses such as low temperature,hormone response,and growth and development were identified.Through the collinearity analysis of interspecific Trihelix transcription factors of model plants Arabidopsis thaliana and ginseng,19 collinear gene pairs were found between A.thaliana and ginseng,and no collinear gene pairs existed on chromosomes 3,6,and 12 only.qRT-PCR analysis showed that the expression of GWHGBEIJ010320.1 was significantly up-regulated under low temperature stress,a significant response to low temperature stress.This study lays a foundation for further research on the role of the Trihelix transcription factor of ginseng in abiotic stress,as well as the growth and development of ginseng.
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