Chemical constituents from stem bark of Aquilaria sinensis and their antibacterial activity against Helicobacter pylori
The stem bark of Aquilaria sinensis(Thymelaeaceae),with the local name of"Li-Wa-Zi-Xing",is used in traditional Yi medicine for treating chronic gastritis and other diseases.However,its active ingredients remain currently unknown.In this study,Helicobacter pylori(Hp)is used in anti-bacterial experiments to test the active compounds derived from A.sinensis stem bark.Nineteen compounds were isolated from the stem bark of A.sinensis by column chromatography,high-performance liquid chromatography,recrystallization,etc.Aquilaridiester(1)is a new lignan.The other eighteen compounds were reported before,including docosyl caffeate(2),6-hydroxy-2-[2-(4-methoxyphenyl)ethyl]-4H-1-benzopyran-4-one(3),qinanone A(4),6-hydroxy-2-(2-phenylethyl)chromone(5),6-hydroxy-2-[2-(3-hydroxy-4-methoxyphenyl)ethyl]-4H-1-benzopyran-4-one(6),6-hydroxy-2-[2-(3-methoxy-4-hydroxyphenyl)ethyl]-4H-1-benzopyran-4-one(7),6-hydroxy-2-[2-(3,4-dimethoxyphenyl)ethyl]chromone(8),6-hydroxy-2-[(1E)-2-(4-hydroxy-3-methoxyphenyl)ethenyl]-4H-1-benzopyran-4-one(9),genkwanin(10),5-hydroxy-2-(4-hydroxy-3,5-dimethoxyphenyl)-7-methoxy-4H-1-benzopyran-4-one(11),3-hydroxy-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-propanone(12),(+)-syringaresinol(13),zhebeiresinol(14),aquilarin A(15),caruilignan D(16),(-)-ficusal(17),pistaciamide(18),and protocatechuic acid(19).The anti-bacterial results show that compounds 2-7,10-11,and 13 have inhibitory activity against Hp.Among them,6-hydroxy-2-(2-phenylethyl)chromone(5)and 6-hydroxy-2-[2-(3-methoxy-4-hydroxyphenyl)ethyl]-4H-benzopyran-4-one(7)have superior inhibitory effects on Hp to others,with the same minimum inhibitory concentration(MIC)of 6.25μmol·L-1.The 2-(2-phenylethyl)chromones are the major active ingredients in A.sinensis stem bark.
Aquilairastem bark of Aquilaria sinensisgastritisHelicobacter pylori2-(2-phenylethyl)chromone
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