黄精水提物对慢性阻塞性肺疾病模型大鼠的作用研究
Effect of Polygonati Rhizoma aqueous extract on chronic obstructive pulmonary disease in rats
李进 1苏洁 1董英杰 1周衡朴 1高帆 1陈素红 2吕圭源1
作者信息
- 1. 浙江中医药大学 药学院,浙江 杭州 310053
- 2. 浙江工业大学 药学院,浙江 杭州 310014
- 折叠
摘要
探究黄精水提物对慢性阻塞性肺疾病(COPD)模型大鼠作用及可能的机制.选取 48 只SD大鼠随机分成正常组,模型组,玉屏风颗粒组(1.5 g·kg-1),黄精水提物低、中、高剂量组(0.25、0.5、1 g·kg-1),采用香烟烟雾熏吸法 8 周建立COPD大鼠模型后,给药 4 周,检测大鼠抓力、粪便含水率;取肺组织计算肺指数;酶联免疫吸附测定(ELISA)法检测大鼠肺组织中白细胞介素(IL)-6、肿瘤坏死因子(TNF)-α含量;苏木素-伊红(HE)染色、Masson染色法检测肺组织病理变化;流式细胞术检测外周血T淋巴细胞及其亚群;免疫荧光法和Western blot法检测肺组织中Toll样受体 4(TLR4)、核转录因子-kappaB(NF-κB)、磷酸化NF-κB(p-NF-κB)、核因子κB抑制蛋白α(IκBα)、磷酸化IκBα(p-IκBα)、IL-6 和TNF-α蛋白表达.结果显示,经黄精水提物干预后,各组大鼠粪便含水率均显著降低,抓力均显著增加,肺组织炎症浸润、纤维化减少;大鼠外周血中T细胞、Th细胞比例及Th/Tc比值显著增加,Tc细胞比例显著下降;肺组织中TLR4、p-NF-κB/NF-κB、p-IκBα/IκBα、IL-6、TNF-α表达水平均显著降低.综上,黄精水提物能改善COPD模型大鼠肺组织病理损伤,其机制与抑制TLR4/NF-κB信号通路,减少炎症因子产生有关.
Abstract
This study aimed to investigate the effects and potential mechanism of Polygonati Rhizoma aqueous extract on chronic obstructive pulmonary disease(COPD)in rats.Forty-eight Sprague-Dawley rats were randomly assigned to the normal,model,Yupingfeng Granules(1.5 g·kg-1),and low-,medium-,and high-dose(0.25,0.5,and 1 g·kg-1,respectively)Polygonati Rhizoma aqueous extract groups.The rat model of COPD was established by cigarette smoke inhalation for 8 weeks,and then the modeled rats received corresponding treatment for 4 weeks.The grip strength and fecal moisture content were measured,and the lung index was calculated.Enzyme-linked immunosorbent assay(ELISA)was employed to determine the levels of interleukin(IL)-6 and tumor necrosis factor(TNF)-α in the lung tissue.Hematoxylin-eosin(HE)staining and Masson staining were performed to assess the pathological changes in the lung tissue.Flow cytometry was used to analyze T lymphocytes and their subpopulations in the peripheral blood,and the immunofluorescence assay and Western blot were employed to measure the protein levels of Toll-like receptor 4(TLR4),phosphorylated nuclear factor-kappaB(p-NF-κB),NF-κB,phosphorylated inhibitory kappa B-α(p-IκBα),IκBα,IL-6,and TNF-α in the lung tissue.The results indicated that the treatment with Polygonati Rhizoma aqueous extract significantly reduced the fecal moisture content,enhanced the grip strength,and inhibited inflammatory infiltration and fibrosis in the lung tissue.The treatment increased the Th/Tc ratio and Th cell proportion and decreased the Tc cell proportion in the peripheral blood.Furthermore,the treatment down-regulated the expression levels of TLR4,IL-6,and TNF-α and the p-NF-κB/NF-κB and p-IκBα/IκBα ratios in the lung tissue.In conclusion,Polygonati Rhizoma aqueous extract can ameliorate lung tissue damage in the rat model of COPD by inhibiting the TLR4/NF-κB signaling pathway and the production of inflammatory mediators.
关键词
黄精水提物/慢性阻塞性肺疾病(COPD)/炎症/TLR4/NF-κB信号通路Key words
Polygonati Rhizoma aqueous extract/chronic obstructive pulmonary disease(COPD)/inflammation/TLR4/NF-κB signaling pathway引用本文复制引用
基金项目
国家重点研发计划项目(2017YFC1702203)
浙江省重点研发计划项目(2020C04020)
浙江省重点实验室项目(2012E10002)
出版年
2024
NETL
NSTL
万方数据