艾草R2R3-MYB转录因子家族成员的鉴定及表达分析

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中文摘要：MYB(v-myb avian myeloblastosis viral oncogene homolog)家族转录因子是高等植物转录因子中数目最多的一类基因家族成员，可分为 4 个亚家族，其中R2R3-MYB是最常见的亚家族类型。R2R3-MYB类转录因子广泛参与植物的器官发育和次生代谢产物生物合成的调控。为研究R2R3-MYB家族转录因子在艾叶黄酮合成和腺毛发育中发挥的作用，该研究基于艾的全基因组数据筛选、鉴定出 92 个R2R3-MYB转录因子，并利用生物信息学预测其潜在功能。结果表明，92 个转录因子的氨基酸长度为 168～547 aa，相对分子质量为 19。6～60。5 kDa，均为亲水性蛋白。亚细胞定位分析表明，89 个AaMYB 蛋白定位结果为细胞核，3 个蛋白同时定位于细胞核和细胞质中。依据拟南芥R2R3-MYB家族分类，92 个艾R2R3-MYB蛋白分为 26 个亚家族，同一亚族基因结构基本类似。顺式作用元件预测结果表明光响应元件、茉莉酸甲酯元件和脱落酸元件在R2R3-MYB基因启动子区域分布广泛。转录组表达分析结果显示，AaMYB60、AaMYB63 和AaMYB86 在叶片中的表达均高于茎和根中的表达，说明这 3 个转录因子主要在叶片中发挥作用。此外，利用系统进化分析筛选出 5 个参与艾叶黄酮生物合成或腺毛发育的候选R2R3-MYB转录因子。该研究可为后期艾的优良品种选育和种质创新等提供重要的基因资源。

外文标题：Identification and expression analysis of members of R2R3-MYB transcription factors family in Artemisia argyi

外文摘要：The MYB(v-myb avian myeloblastosis viral oncogene homolog)family of transcription factors is the largest class of genes among higher plant transcription factors,which can be divided into four subfamilies,with the R2R3-MYB being the most common subfamily type.R2R3-MYB transcription factors are widely involved in the regulation of organ development and secondary metabolite biosynthesis in plants.To investigate the role of R2R3-MYB family transcription factors in the synthesis of flavonoids and glandular trichome development in Artemisia argyi,this study screened and identified 92 R2R3-MYB transcription factors based on the whole genome data of A.argyi,and predicted their potential functions based on bioinformatics.The results showed that the amino acid lengths of the 92 transcription factors ranged from 168 to 547 aa,with relative molecular weights ranging from 19.6 to 60.5 kDa,all of which were hydrophilic proteins.Subcellular localization analysis showed that 89 AaMYB proteins were located in the nucleus,while three proteins were simultaneously located in the nucleus and cytoplasm.According to the classification of Arabidopsis R2R3-MYB family,the 92 A.argyi R2R3-MYB proteins were divided into 26 subfamilies,with similar gene structures within the same subfamily.Cis-acting element prediction results showed that light-responsive elements,methyl jasmonate elements,and abscisic acid elements were widely distributed in the promoter regions of R2R3-MYB genes.Transcriptome expression analysis results showed that the expression of AaMYB60,AaMYB63,and AaMYB86 in leaves was higher than that in stems and roots,indicating that these three transcription factors mainly function in leaves.Additionally,five candidate R2R3-MYB transcription factors involved in A.argyi flavonoid biosynthesis or glandular trichome development were selected through phylogenetic analysis.This study provides important genetic resources for the breeding of superior varieties and germplasm innovation of A.argyi in the future.

外文关键词：

Artemisia argyiR2R3-MYB transcription factorgenomebioinformaticssystematic evolution

作者：

崔占虎、李淑娇、满永宏、李明杰、古力、张重义、李孟芝、黄显章、王宇卿

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作者单位：

南阳医学高等专科学校河南省中药效应物质与质量控制研究中医药重点实验室南阳道地药材(艾草)研究院,河南南阳 473061

福建农林大学,福建福州 350002

南阳理工学院河南省张仲景方药与免疫调节重点实验室,河南南阳 473004

关键词：

艾 R2R3-MYB转录因子基因组生物信息学系统进化

基金：

国家自然科学基金项目南阳市重大科技专项中央本级重大增减支项目财政部和农业农村部国家现代农业产业技术体系项目河南省高等学校重点科研项目南阳市科技攻关项目南阳市科技攻关项目南阳市科技攻关项目

项目编号：

822045652023ZDZX1052060302CARS-2123B35000722KJGG10623KJGG16923KJGG170

出版年：

2024

DOI：

10.19540/j.cnki.cjcmm.20240415.103

中国中药杂志

中国药学会

中国中药杂志

CSTPCD北大核心

影响因子：1.718

ISSN：1001-5302

年,卷(期)：2024.49(16)