Effect of Sijunzi Decoction on intestinal barrier of type 2 diabetic mice
This study aims to explore the improvement effect of Sijunzi Decoction on intestinal barrier in diabetic mice.A type 2 diabetes mellitus(T2DM)model was established in C57BL/6J mice by feeding them with high-sugar and high-fat diet combined with streptozotocin(STZ).The T2DM mice were randomly divided into a control group,a T2DM group,a donepezil(DON)group,a rosiglitazone(RGZ)group,and Sijunzi Decoction groups(7.5,15,and 30 g·kg-1),and orally administered for six weeks.The body weight and fasting plasma glucose(FBG)of mice were recorded.Fasting plasma insulin(FINS)and insulin resistance index(HOMA-IR)were observed to assess insulin resistance(IR).Intestinal flora and levels of serotonin(5-HT),lipopolysaccharide(LPS),and short-chain fatty acids(SCFAs)in serum were analyzed.Changes in colonic structure and tight junction proteins occludin,claudin-1,and ZO-1 were observed through HE staining and immunohistochemistry.Spontaneous alternation test was conducted to observe the effect on spatial memory ability.Compared with the results in the control group,FBG and HOMA-IR in the T2DM group were significantly increased(P<0.01);species richness index(Sobs index),Shannon diversity index(Shannon index),and species abundance estimate index(Chao index)were decreased;LPS was significantly increased(P<0.001),while the levels of 5-HT,SCFAs,occludin,claudin-1,and ZO-1 were significantly decreased(P<0.01),indicating impaired colonic barrier function;spontaneous alternation accuracy was significantly decreased(P<0.05).After 6 weeks of Sijunzi Decoction treatment,compared with the results in the T2DM group,FBG and HOMA-IR in the Sijunzi Decoction 15 g·kg-1 group were significantly decreased(P<0.01);Sobs index,Shannon index,and Chao index were increased;LPS was significantly decreased(P<0.01),while the levels of 5-HT,SCFAs,occludin,claudin-1,and ZO-1 were significantly increased(P<0.05),indicating improved colonic barrier function;spontaneous alternation accuracy was increased(P<0.001).In conclusion,Sijunzi Decoction has the effect of improving intestinal barrier in diabetic mice.
NETL
NSTL
万方数据
