首页|基于多元统计分析的不同产地枸杞子对碘酸钠诱导的视网膜变性小鼠的干预作用比较研究

基于多元统计分析的不同产地枸杞子对碘酸钠诱导的视网膜变性小鼠的干预作用比较研究

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基于视网膜变性(retinal degeneration diseases，RDD)小鼠模型，采用多元统计分析方法比较不同产地枸杞子的视网膜保护作用。通过腹腔注射碘酸钠(NaIO3)构建小鼠RDD小鼠模型，采用双门黑白箱和TUNEL测定评估视觉功能和视网膜细胞凋亡情况;采用眼底光学相干断层扫描(OCT)检测视网膜厚度并进行眼底照相，测定血清和眼球中氧化应激、炎症和血管生成标志物的水平。采用层次聚类分析、主成分分析和偏最小二乘法判别分析进行不同产地枸杞子对小鼠RDD模型视网膜保护作用的综合比较。结果显示，不同产地枸杞子提取物均可逆转NaIO3 诱导的视觉及视网膜损伤和细胞凋亡，改善小鼠体内氧化应激水平及炎症因子和血管生成标志物的表达。基于 17 个药效学指标进行多元统计分析后发现，产于宁夏的枸杞子提取物相较于其余 4 个产地的受试样品呈现更好的RDD治疗效应。研究结果可为防治RDD的枸杞子优势产地选择提供科学依据。
Comparison on effects of Lycii Fructus from different origins on NaIO3-induced retinal degenerative diseases in mice by multivariate statistical analysis
The multivariate statistical analysis was performed to compare the therapeutic effects of Lycii Fructus from different origins on the retinal degenerative diseases(RDD)in mice.The mouse model of RDD was established by intraperitoneal injection of NaIO3,and the visual function and retinal apoptosis were assessed by dark-light transition and TUNEL assay.Retinal thickness was measured by fundus optical coherence tomography(OCT),and the levels of antioxidant,inflammatory,and angiogenic markers in the serum and eyeball were determined.The therapeutic effects were compared by hierarchical cluster analysis,principal component analysis,and partial least squares-discriminant analysis.The results showed that the extracts of Lycii Fructus from different origins reversed NaIO3-induced visual damage and retinal apoptosis,reduced oxidative stress,and restored the expression of inflammatory mediators and angiogenic markers in mice.The multivariate statistical analysis based on 17 pharmacodynamic indices suggested that the extract of Lycii Fructus from Ningxia demonstrated better therapeutic effects on RDD than the samples from the other four origins.The results of this study provide a scientific basis for the selection of the advantageous production region of Lycii Fructus for the prevention and treatment of RDD.

Lycii Fructusoptic nerve protectionretinal degenerative diseasemultivariate statistical analysis

陈鑫、郭盛、王杰美、李洁、倪亮、康宏杰、钱大玮、段金廒

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南京中医药大学 中药资源产业化与方剂创新药物国家地方联合工程研究中心/江苏省中药资源产业化过程协同创新中心/国家中医药管理局中药资源循环利用重点研究室,江苏 南京 210023

南京中医药大学附属医院,江苏 南京 210029

宁夏枸杞创新中心,宁夏 银川 750002

枸杞子 视神经保护 视网膜变性 多元统计分析

宁夏重点研发计划重点项目宁夏重点研发计划重点项目国家自然科学基金区域创新发展联合基金重点项目江苏省重点研发计划项目江苏省重点研发计划项目中央本级重大增减支项目

2021BEF020102021BEF02009U21A20408BE2021907BE20219122060302

2024

10.19540/j.cnki.cjcmm.20240414.103

中国中药杂志
中国药学会

中国中药杂志

CSTPCD北大核心
影响因子：1.718
ISSN：1001-5302
年,卷(期)：2024.49(16)