Chemical components and in vitro anti-aging activity of Yangxue Qingnao Wan based on UPLC-Q-TOF-MS/MS
The main chemical components of Yangxue Qingnao Wan(YXQNW)were analyzed and identified by ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS).According to the mass spectrometry information,Mass Hunter 10.0 analysis software was used to compare the collected quasi-molecular ion peaks and secondary fragment ions with literature and reference substances.A total of 131 compounds were identified from YXQNW,including 11 phenylpropanoids,11 flavonoids,42 nitrogen-containing compounds,12 terpenoids,17 phthalides,23 quinones,and 15 other compounds.The anti-aging activity of YXQNW and six compounds from YXQNW,including rosmarinic acid,gallic acid,rutin,umbelliferone,hyperoside,and vanillic acid,were evaluated by D-galactose(D-gal)-induced HT22 cell senescence model.The effects of the compounds on HT22 cell damage and individual cell proliferation ability were observed from overall and individual perspectives by the Beyo ClickTM EdU-555 cell proliferation kit,and apoptosis was detected by the Annexin V-FITC/PI double staining apoptosis detection kit.Finally,the anti-aging effect of the compounds was tested by a cell senescence β-galactosidase staining kit.This study provides a more comprehensive analysis of the chemical components of YXQNW and evaluates its anti-aging effect,which will provide a scientific basis for basic research on the efficacy of YXQNW for the treatment of various neurological diseases,such as Alzheimer's disease(AD),headache,and memory loss.
Yangxue Qingnao Wanultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)component analysisanti-aging activity
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