Bioactive secondary metabolites from an endophytic fungus Aspergillus sp.CCH-1E from Catharanthus roseus
This study focused on the bioactive secondary metabolites of an endophytic fungus Aspergillus sp.CCH-1E from Catha-ranthus roseus.The secondary metabolites from Aspergillus sp.CCH-1E were isolated by using various chromatographic methods[such as normal-phase and reversed-phase chromatography and high-performance liquid chromatography(HPLC)],and their structures were identified by various spectroscopic methods[e.g.,ultraviolet(UV)spectroscopy,infrared(IR)spectroscopy,nuclear magnetic reso-nance(NMR)spectroscopy,and high-resolution electrospray ionization mass spectrometry(HR-ESI-MS)].Twelve compounds were yielded and identified from Aspergillus sp.CCH-1E,which are chermesinone H(1),chermesinone I(2),chermesinone B(3),8,11-didehydrochermesinone B(4),chermesinone C(5),chermesinone A(6),chevalone B(7),barbacenic acid(8),3,6,8-tri-hydroxy-3,5,7-trimethyl-3,4-dihydroisocoumarin(9),5-hydroxy-2-methoxy-7-methyl-1,4-naphthoquinone(10),1-hydroxy-6,8-dim-ethoxy-3-methylanthracene-9,10-dione(11),and 7-drimen-9α,11,12-triol(12).Among them,compounds 1 and 2 are new com-pounds.The growth inhibition effects of all compounds were evaluated against non-small cell lung cancer cell lines A549 and NCI-H1650,as well as human cervical cancer cell line HeLa by using methylthiazolyldiphenyl-tetrazolium bromide(MTT).Compound 7 significantly inhibited the growth of three tumor cells with the IC50 values of 1.22-2.43 μmol·L-1,respectively.Compounds 1-6 showed moderate cell growth inhibition with the IC50 values of 16.24-35.28 µmol·L-1.
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