该研究建立了一种超高液相色谱-串联质谱(UPLC-MS/MS)分析方法，测定给予舒肝宁注射液或黄芩提取物后5种指标成分在大鼠组织器官中的含量，研究二者经时动态变化规律和差异，探讨黄芩配伍前后对5种成分组织分布的影响。大鼠尾静脉注射舒肝宁注射液或黄芩提取物后，分别于0。17、0。5、1、2 h 4个时间点采集心、肝、脾、肺、肾、胃、肠、脑组织样品，采用UPLC-MS/MS检测5种指标成分(黄芩苷、黄芩素、千层纸素A、千层纸素A-7-O-β-D-葡萄糖醛酸苷、野黄芩苷)在2组大鼠组织中的浓度。结果表明建立的UPLC-MS/MS测定方法简便快速且专属稳定。组织分布结果表明，给予舒肝宁注射液和黄芩提取物后，5种指标成分在体内分布广泛且存在一定差异，2组中黄芩苷、黄芩素和千层纸素A在肾和肝组织、千层纸素A-7-O-β-D-葡萄糖醛酸苷在肾和脑组织以及野黄芩苷在肾和心组织中分布较多。配伍组方后在心、肝、肾、肠组织中黄芩苷和肝、肾组织中黄芩素以及肺组织中千层纸素A含量均显著增加。研究表明，舒肝宁注射液与黄芩提取物中的5种成分在大鼠体内分布广泛且无蓄积情况，黄芩配伍组方后可增加有效成分在大鼠体内的分布，为从体内过程角度阐释舒肝宁注射液的配伍合理性提供依据。
Comparison on tissue distribution of five components in Shuganning Injection and Scutellariae Radix extract by UPLC-MS/MS
This study established an ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method to determine the content of five index components in rat tissues and organs after administration of Shuganning Injection or Scute-llariae Radix extract.The dynamic changes and differences of the distribution of the five index components over time between the two groups were studied,and the effects of Scutellariae Radix alone or in combination with other medicines on the tissue distribution of the five components were explored.After Shuganning Injection or Scutellariae Radix extract was injected into the tail vein of rats,the heart,liver,spleen,lung,kidney,stomach,intestine,and brain tissue samples were collected at four time points of 0.17,0.5,1,and 2 h,respectively.UPLC-MS/MS was employed to measure the concentrations of the five index components(baicalin,baicalein,oroxylin A,oroxylin A-7-O-β-D-glucuronide,and scutellarin)in the samples of the two groups.The results showed that the established method was simple,fast,and exclusively stable.After the administration of Shuganning Injection and Scutellariae Radix extract,the five index components presented wide distribution and had differences in vivo.The two groups showcased abundant distribution of baica-lin,baicalein,and oroxylin A in the kidney and liver,oroxylin A-7-O-β-D-glucuronide in the kidney and brain,and scutellarin in the kidney and heart.The content of baicalin in the heart,liver,kidney,and intestine,baicalein in the liver and kidney,and oroxylin A in the lung after administration of Shuganning Injection(Scutellariae Radix in combination with other medicines)was significantly higher than that after administration of Scutellariae Radix extract.The results of this study suggested that the five components of Shuganning Injection and Scutellariae Radix extract demonstrated wide distribution without accumulation in rats.The combination of Scutellariae Radix with other medicines can increase the distribution of active components in rats,which provided a basis for explaining the rationality of the compatibility of Shuganning Injection from in vivo processes.
Shuganning InjectionScutellariae Radix extractUPLC-MS/MStissue distribution
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