Study on differences in metabolism and transcription of ginseng seeds after morphological post ripening by space flight
To explore the difference in metabolism and transcription between seeds experiencing space flight and ground seeds after morphological post ripening,this study utilized ginseng seeds experiencing space flight and ground seeds as materials.Metabolomics and transcriptomics analyses were conducted using ultra-high performance liquid chromatography-mass spectrometry(UPLC-MS)and high-throughput transcriptome sequencing(RNA-seq)technologies,so as to identify differential terpenoid metabolites,differential en-dogenous hormones,and differentially expressed genes.The results showed that through metabolomics analysis,a total of 22 differential terpenoid metabolites were identified in the experimental and control groups,including chikusetsusaponin FK7,ginsenoside F2,ginseno-side K,majoroside R1,ginsenoside Re5,12-hydroxyabietic acid,etc;through transcriptomics analysis,15 differential terpenoid me-tabolism-related differentially expressed genes were identified in the experimental and control groups,including FCase,AACT,PMK,etc,and these genes were integrated into the pathway based on the MEP and MVA.At the same time,genes related to the seed germi-nation process such as LEC2,PIPK,PUF,REC,QUD,and PIR were screened.In the detection of endogenous hormones,a total of 17 differential hormone metabolites were obtained,including IAA,CK,JA,ABA,GA,and SA.Among them,the content of CK in-creased in the SP group,while the content of GA and SA decreased in the SP group.Genes related to CK,GA,and SA were also screened.This study laid the foundation for further exploration of the biosynthesis of terpenoid metabolites in ginseng seeds and the key endogenous hormones and enzyme genes involved in the seed germination process,and it provided a reference for the application of space flight mutagenesis technology in ginseng.
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