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半夏倍性调查及全基因组Survey分析

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半夏Pinellia ternata 在我国分布广泛，以块茎入药，有燥湿化痰等功效。其倍性复杂，且目前未获得全基因组图谱，限制了分子辅助育种及多组学的深入研究。该研究首先基于流式细胞术对来自我国16个省市的144株不同表型半夏进行基因组大小评估，发现其均值为(7。97±0。43)Gb，最高及最低值分别为 7。06、9。21 Gb。此外，云南半夏基因组大小相对偏低[(7。24±0。09)Gb]，而采自湖北仙桃的紫皮半夏个体间基因组大小较为一致[(8。07±0。04)Gb]。进一步通过根尖染色体计数发现，流式细胞测定结果为8。18 Gb的半夏(2n=8x=104)为八倍体，其余均为非整倍体植株。采用Illumina技术完成该八倍体的75×深度，使用3种方法完成测序结果的K-mer分析。该八倍体半夏基因组大小为 7 367。082～7 541。213 Mb，重复序列比率为 91。3%±1。7%，杂合率为1。68%，是一种复杂基因组。该研究开展半夏群体的倍性调查及特定倍性株系的全基因组Survey分析，可为其全基因组测序和组装奠定基础，同时为倍性复杂、纯系缺乏、植株矮小的草药提供可参考的全基因组调查分析策略。
Ploidy and whole genome Survey of Pinellia ternata
Pinellia ternata,a widely distributed species in China,has been used as a herbal medicine for centuries,with the effects of drying dampness and resolving phlegm.However,its complex ploidy and lack of whole-genome map limit in-depth research on molecular-assisted breeding and multi-omics.In this study,flow cytometry was employed to evaluate the genome sizes of 144 P.ternata plants with different phenotypes collected from 16 geographical locations in China,which revealed the average,maximum,and minimum genome sizes of(7.97±0.43)Gb,7.06 Gb,and 9.21 Gb,respectively.In addition,the plants from Yunnan province had small genomes(7.24±0.09)Gb,while those with purple skin from Xiantao,Hubei presented similar genome sizes(8.07±0.04)Gb.The plant with a flow cytometry result of 8.18 Gb(2n=8x=104)was identified as an octoploid by the root tip chromosome counting method.Illumina was employed to sequence the octoploid P.ternata at a depth of 75×,and three mainstream methods were used for K-mer analysis.The estimated genome size range of this plant was 7 367.082-7 541.213 Mb,and the heterozygosity rate and repeated sequence ratio were 1.68%and 91.3%±1.7%,respectively.A ploidy Survey of P.ternata populations and a whole-genome survey of specific ploidy strains were carried out,which laid a foundation for whole-genome sequencing and assembly.Meanwhile,this study provided a reference strategy of whole genome Survey for herbal species with complex ploidy,lack of pure lines,and dwarf plants.

flow cytometrygenomic investigationchromosome counting in root tipoctoploidplant tissue culture

裴艺菲、刘姿怡、赵贵萍、陈莹莹、张翔宇、陈晓芳、冯雪、李西文

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中国中医科学院 中药研究所,北京 100700

毕节市中药研究所,贵州 毕节 551700

毕节医学高等专科学校,贵州 毕节 551700

流式细胞术 基因组调查 根尖染色体计数 八倍体 植物组织培养

毕节市科技局科技计划"揭榜挂帅"项目中国中医科学院科技创新工程项目中央级公益性科研院所基本科研业务费专项中央级公益性科研院所基本科研业务费专项中央级公益性科研院所基本科研业务费专项国家重点研发计划项目毕节市科技创新平台及人才团队"毕节市健康产业重点实验室"项目

毕科合重大专项字20212号CI2021A04106ZXKT21026ZZ15-YQ-033ZXKT230042019YFC1710600毕科合202366号:BJZDSYS2024-05

2024

10.19540/j.cnki.cjcmm.20240714.103

中国中药杂志
中国药学会

中国中药杂志

CSTPCD北大核心
影响因子：1.718
ISSN：1001-5302
年,卷(期)：2024.49(19)