Chemical constituents from petroleum ether fraction of Artemisia stolonifera
The chemical constituents in the petroleum ether fraction of Artemisia stolonifera were investigated.Twenty compounds(1-20)were isolated from the petroleum ether fraction of A.stolonifera by silica gel,Sephadex LH-20,and ODS chromatography and semi-preparative HPLC.Based on physicochemical properties and spectral data,the structures of these compounds were identified as(7S,9S,10S)-9-hydroxy-7-allyl acetate-1-one(1),1β,4β,7α-trihydroxy-8,9-eudesmene(2),oplodiol(3),10α-hydroxycadin-4-en-15-al(4),loliolide(5),vanillin(6),vanillic acid(7),methoxyeugenol(8),ethyl 4-hydroxy-3-methoxybenzoate(9),ethyl 4-hydroxybenzoate(10),(E)-4-hydroxy-3-methyl-2-butenyl benzoate(11),(3R,6R,7E)-3-hydroxy-4,7-megastigmadien-9-one(12),hydroxydihydrobovolide(5-hydroxy-3,4-dimethy-5-pentyl-2(5H)-furanone(13),(R)-2-ethylhexyl 2H-1,2,3-triazole-4-carboxylate(14),eriodictyol-7,3′-dimethyl ether(15),progesterone(16),spinasterol(17),schottenol(18),α-tocospiro A(19),and 3-[3′-(nona-1″-en-3″,5″,7″-trynyl)oxiran-2′-yl]propan-2-ol(20).Compound 1 was a new sesquiterpenoid,and compounds 2-5 and 7-20 were isolated from this plant for the first time.The antitumor activities of the compounds were studied.The results showed that compounds 16 and 19 displayed cytotoxic activity against MCF-R7 and B16 cells,with the IC50 values of(20.94±1.72)and(77.45±0.35)μmol·L-1 against MCF-7 cells and(33.89±2.98)and(23.42±0.31)μmol·L-1 against B16 cells,respectively.Compounds 11,15,and 20 had cytotoxic activity against B16 cells,with the IC50 values of(88.13±6.38),(68.05±10.28),and(58.89±10.29)μmol·L-1,respectively.
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