Rose roxburghii polysaccharide-induced apoptosis of prostate cancer DU145 cells by inhibiting PI3K/Akt/mTOR pathway and antioxidant effects
Based on the signaling pathway of phosphoinositide 3-kinase(PI3K)/protein kinase B(Akt)/mammalian target of rapa-mycin(mTOR),pathway-related phosphatase and tensin homolog(PTEN),B-cell lymphoma-2(Bcl-2),and Bcl-2-associated X pro-tein(BAX),the mechanism of Rose roxburghii polysaccharides in inhibiting proliferation and inducing apoptosis of prostate cancer DU145 cells was explored,as well as the antioxidant activity of R.roxburghii polysaccharides.Prostate cancer DU145 cells were trea-ted with different concentrations of R.roxburghii polysaccharides.The effect of R.roxburghii polysaccharides on the proliferation of DU145 cells was detected by the CCK-8 method,and the effect of R.roxburghii polysaccharides on the migration of DU145 cells was detected by cell scratch test.In addition,a Transwell assay was conducted to detect the effect of R.roxburghii polysaccharides on the invasion ability of DU145 cells.The apoptosis of DU145 cells induced by R.roxburghii polysaccharides was detected by flow cytome-try.Real-time PCR and Western blot were used to detect the effects of R.roxburghii polysaccharides on the mRNA and protein expres-sion levels of PI3K,Akt,mTOR,PTEN,BAX,and Bcl-2 in DU145 cells.DPPH and ABTS assays were used to determine the an-tioxidant activity of R.roxburghii polysaccharides in vitro.The results showed that R.roxburghii polysaccharides inhibited the prolife-ration,migration,and invasion of DU145 cells.Flow cytometry analysis showed that compared with that of the control group,the apop-tosis rate of DU145 cells in groups treated with R.roxburghii polysaccharides was increased with the increase in the concentration of R.roxburghii polysaccharides,and its inhibition was positively correlated with the concentration of R.roxburghii polysaccharides.R.roxburghii polysaccharides inhibited the PI3K/Akt/mTOR pathway and induced apoptosis of DU145 cells by up-regulating the pro-tein and gene expression of PTEN and BAX and down-regulating the expression of Akt,PI3K,mTOR,and Bcl-2.R.roxburghii poly-saccharides could scavenge ABTS and DPPH radicals to a certain extent,suggesting that R.roxburghii polysaccharides may induce the apoptosis of DU145 cells by scavenging intracellular ROS.R.roxburghii polysaccharides may inhibit the proliferation of DU145 cells and induce its apoptosis by inhibiting the PI3K/Akt/mTOR pathway and clearing intracellular ROS.
Rose roxburghii polysaccharidePI3K/Akt/mTOR pathwayprostate cancer DU145 cellsapoptosisantioxidant
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