Construction of black-bone silky fowl (Gallus gallus domesticus) families based on genetic diversity
Black-bone silky fowls (Gallus gallus domesticus) have a long history of medicinal use,with the origin in Taihe county,Jiangxi province.The unclear family composition,inbreeding rate,and effective population size were inconducive to the resource conservation or breed improvement of black-bone silky fowls.A genome-wide analysis was performed to evaluate the genetic diversity of 80 black-bone silky fowls from random mating in three farms in 2021 in terms of minor allele frequency(MAF),expected heterozygosity (He),observed heterozygosity(Ho),effective population size(Ne),and runs of homozygosity (ROH).The results showed that a total of 9641411 SNPs were passed quality control from 80 black-bone silky fowls.The polymorphic marker ratio,expected heterozygosity,and observed heterozygosity in this population were 0.81,0.34,and 0.33,respectively,and the average IBS genetic distance of black-bone silky fowls was 0.38±0.0082.A total of 15969 ROHs were identified,with the average length of 0.73 Mb,and most of the ROHs were 0～2 Mb.According to inbreeding ROHs,the average inbreeding coefficient was 0.1509.The high-frequency ROH regions harbored 424 genes,including two genes,SLC13A4 and DDX6,associated with pigmentation.The results showed that the genetic relationship between individuals of black-bone silky fowls was far.The population had experienced a strong decline in size in recent decades,and the inbreeding degree was high.According to the results,an appropriate family construction scheme was proposed for resource conservation and breeding work of black-bone silky fowls.
万方数据
维普
