摘要
综合运用多种现代色谱分离方法从薰鲁香中分离得到10个化合物,通过HR-ESI-MS、IR、UV、NMR、量子化学计算核磁共振参数(qcc-NMR)等技术和文献对比,分别鉴定为17β-hydroxy-28-norolean-18-en-3-one(1)、28-norolean-12,17-dien-3,11-dione(2)、28-norolean-16,18-dien-3-one(3)、(24Z)-26-hydroxy-7,24-dientirucalla-3-one(4)、masticadienonic acid(5)、mastica-dienolic acid(6)、erythrodiol(7)、3β,28-dihydroxyoleana-11,13(18)-diene(8)、3-oxo-olean-9(11),12-dien-28-oic acid(9)、12-oleane-3,11-dione(10).其中,化合物1为新化合物,化合物2为新天然产物,化合物1～2、4、7～9均为首次从薰鲁香中分离得到.化合物1可显著抑制脂多糖(LPS)诱导的小鼠单核巨噬细胞RAW264.7中NO的产生,IC50为(7.44±0.49)μmol·L-1,强于阳性对照地塞米松[dexamethasone,IC50为(9.93±1.17)μmol·L-1],化合物2～3、5、9～10显示出一定的抑制效果,IC50在14.82～28.82μmol·L-1;化合物4～5、7可显著抑制人结肠腺癌细胞SW480的生长,IC50分别为(16.13±1.69)、(27.40±1.81)、(10.01±0.10)μmol·L-1.
Abstract
Ten compounds were separated by various modern chromatographic methods from mastic.They were identified by HR-ESI-MS,IR,UV,NMR,quantum chemical calculation of NMR (qcc-NMR) and comparison with reported data in literature as 17β-hydroxy-28-norolean-18-en-3-one (1),28-norolean-12,17-dien-3,11-dione (2),28-norolean-16,18-dien-3-one (3),(24Z)-26-hydroxy-7,24-dientirucalla-3-one (4),masticadienonic acid (5)、masticadienolic acid (6)、erythrodiol (7),3β,28-dihydroxyoleana-11,13(18)-diene (8),3-oxo-olean-9(11),12-dien-28-oic acid (9),and 12-oleane-3,11-dione (10).Among them,compound 1 was a novel compound and compound 2 was a novel natural product.Compounds 1-2,4,and 7-9 were isolated from mastic for the first time.Compound 1 significantly inhibited lipopolysaccharide-induced production of nitric oxide in mouse monocyte macrophage RAW264.7 cells with the IC50 of (7.44±0.49) μmol·L-1,which was more potent than the positive control,dexamethasone[IC50=(9.93±1.17) μmol·L-1].Compounds 2-3,5,and 9-10 also showed inhibitory effects,with the IC50 ranging from 14.82 to 28.82 μmol·L-1.Compounds 4-5,and 7 markedly inhibited the growth of human colon adenocarcinoma cells SW480,with the IC50 of (16.13±1.69),(27.40±1.81),and (10.01±0.10) μmol·L-1,respectively.
维普
万方数据