Ten compounds were separated by various modern chromatographic methods from mastic.They were identified by HR-ESI-MS,IR,UV,NMR,quantum chemical calculation of NMR (qcc-NMR) and comparison with reported data in literature as 17β-hydroxy-28-norolean-18-en-3-one (1),28-norolean-12,17-dien-3,11-dione (2),28-norolean-16,18-dien-3-one (3),(24Z)-26-hydroxy-7,24-dientirucalla-3-one (4),masticadienonic acid (5)、masticadienolic acid (6)、erythrodiol (7),3β,28-dihydroxyoleana-11,13(18)-diene (8),3-oxo-olean-9(11),12-dien-28-oic acid (9),and 12-oleane-3,11-dione (10).Among them,compound 1 was a novel compound and compound 2 was a novel natural product.Compounds 1-2,4,and 7-9 were isolated from mastic for the first time.Compound 1 significantly inhibited lipopolysaccharide-induced production of nitric oxide in mouse monocyte macrophage RAW264.7 cells with the IC50 of (7.44±0.49) μmol·L-1,which was more potent than the positive control,dexamethasone[IC50=(9.93±1.17) μmol·L-1].Compounds 2-3,5,and 9-10 also showed inhibitory effects,with the IC50 ranging from 14.82 to 28.82 μmol·L-1.Compounds 4-5,and 7 markedly inhibited the growth of human colon adenocarcinoma cells SW480,with the IC50 of (16.13±1.69),(27.40±1.81),and (10.01±0.10) μmol·L-1,respectively.
masticchemical constituentsnortriterpenoidanti-inflammatoryanti-colorectal cancer
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