Expression and clinical significance of long non-coding RNA differentially expressed genes,micro RNA-181a-5p and autophagy-related protein 5 in primary gouty arthritis
Objective To explore the expression and clinical significance of long non-coding RNA colorectal neoplasia differentially expressed(lncRNA CRNDE),microRNA-181a-5p(miR-181a-5p)and autophagy related 5(ATG5)in the peripheral blood of patients with gouty arthritis(GA)patients.Methods The clinical data,laboratory parameters and peripheral blood samples were collected from 40 patients with acute gout(AG),40 patients with intermittent gout(IG)and 50 healthy subjects(HC).The expression levels of lncRNA CRNDE,miR-181a-5p and ATG5 mRNA were detected by real-time fluorescence quantification(RT-qPCR)and the expression level of ATG5 protein was detected by Western-blot.The expression levels of lncRNA CRNDE,miR-181a-5p,ATG5 mRNA were compared among the three groups and correlated with clinical indices,and a subject operating characteristic curve(ROC)was constructed to assess the value of lncRNA CRNDE,miR-181a-5p,ATG5 mRNA in the diagnosis of gout.Measurements conforming to normal distribution were analyzed using t test or ANOVA,data with non-normal distribution was analyzed using Mann-Whitney U test or Kruskal-Wallis H test,correlation analysis between variables was analyzed using Spearman's analysis,and the diagnostic value of each indicator was analyzed using ROC curve.Results ① The differences in the expression of lncRNA CRNDE,miR-181a-5p,and ATG5 mRNA between the three groups were statistically significant(H=32.12,57.73,68.32,all P＜0.001).Among them,lncRNA CRNDE expression level in the AG group was significantly higher than that in the IG group and healthy control group[61.95(11.39,108.30)×10-3,25.71(15.40,38.40)×10-3,13.80(3.97,23.99)×10-3;Z=-3.24,P=0.001;Z=-5.03,P＜0.001],and the expression level of IG group was higher than that of healthy control group(Z=-3.56,P＜0.001);miR-181a-5p and ATG5 mRNA expression levels in AG group were significantly lower than those in IG group and healthy control group[miR-181a-5p:39.81(31.22,69.38)×10-3,60.74(44.19,90.35)×10-3,121.30(101.50,316.90)×10-3;Z=-3.01,P=0.030;Z=-6.93,P＜0.001.ATG5 mRNA:4.52(2.31,26.63)×10-3,43.63(13.72,102.70)×10-3,153.90(66.62,365.80)×10-3;Z=-5.47,-7.36,all P＜0.001)],which were expressed at lower levels in the IG group than in the healthy controls(Z=-5.25,-4.47,all P＜0.001).The difference of ATG5 protein expression level among the three groups expressed was statistically significant(F=6.24,P=0.030),and the AG group was higher than the healthy control group,and the difference was statistically significant[(0.96±0.13)vs.(0.61±0.04),t=4.25,P=0.013],but the difference between the IG group(0.78±0.15)and the AG group and the HC group was not statistically significant(t=1.51,P=0.206;t=1.85,P=0138).② Spearman correlation analysis showed that lncRNA CRNDE was negatively correlated with the expression levels of miR-181a-5p and ATG5 mRNA in gout patients(r=-0.49,P＜0.001;r=-0.35,P=0.002);miR-181a-5p was positively correlated with ATG5 mRNA expression levels(r=0.64,P＜0.001);lncRNA CRNDE expression level was positively correlated with ESR and WBC(r=0.49,P＜0.001;r=0.43,P=0.001);miR-181a-5p expression level was negatively correlated with ESR and WBC(r=-0.29,P=0.009;r=-0.35,P=0.002),and ATG5 mRNA expression levels were negatively correlated with ESR,WBC,and GR(r=-0.26,P=0.021;r=-0.26,P=0.024;r=-0.27,P=0.021).In the AG group lncRNA CRNDE was positively correlated with ESR and WBC(r=0.36,P=0.022;r=0.36,P=0.026)and miR-181a-5p was negatively correlated with WBC(r=-0.34,P=0.038)③ ROC curve showed that the areas under ROC curve of lncRNA CRNDE,miR-181a-5p and ATG5 mRNA expression levels to predict gout were 0.764,0.875 and 0.864,respectively.The area under ROC curve of gout predicted by the three combined was 0.928.Conclusion lncRNA CRNDE,miR-181a-5p,and ATG5 may be involved in the pathoge-nesis of primary gouty arthritis,and are potential biological parameters for studying the pathogenesis of gout.
Arthritis,goutyRNA,long noncodingMicroRNAsAutophagy-related protein 5
NETL
NSTL
万方数据
