Proteomics in human lymphoblastoid cells after 12C6+ ion irradiation
Objective To investigate the changes of proteomics in human lymphoblastoid cells after 12C6+ ion irradiation.Methods Human lymphoblastoid cells were irradiated with 12C6+ ion of 0,0.1,0.5 and 2.0 Gy at the dose rates of 0.3-0.5 Gy/min.Isobaric tags for relative and absolute quantitation (iTRAQ) was used to analyze the different protein expression in the irradiated cells.Gene ontology(GO),KEGG pathway and protein-protein interaction network analyses were performed.Results In total identified 5 158 kinds of proteins,there were 91,191,and 68 proteins being differentially expressed in the cells irradiated with 0.1,0.5 and 2.0 Gy of 12C6+ ions,respectively,where 11 kinds of proteins were commonly expressed (7 up-regulated and 4 down-regulated) in these three groups compared with control.The analysis with bio-information softwares supposed that these differentially expressed proteins may play important roles in the metabolic process and regulation,protein binding and catalytic activity where fibronectin 1 (FN1) and heat shock protein 1B (HSPA1B) might be the key nodes.Conclusions Irradiation of 12C6+ ions can induce differentially expressed proteins in human lymphoblastoid cells.Analysis on functional roles of SZT2,FN1,and HSPA1B may provide new insight into further mechanistic investigations underlying the molecular biomarkers of 12C6+ ion irradiation.
12C6+ ionHuman lymphoblastoid cellsProteomicsIsobaric tags for relative and absolute quantitation (iTRAQ)
NETL
NSTL
万方数据
维普
