Establishing a three-dimensional model for estimating partial-body irradiation doses based on y-H2AX foci detection
Objective To construct a three-dimensional time-dose-response model for dose estimation and validate its feasibility.Methods Based on a random number table,mice were divided into 0,2,4,6,and 8 Gy groups for whole-body X-ray irradiation,with each group consisting of three mice.Hair follicle cells of whiskers were sampled at 1,6,and 24 h after the irradiation.After immunofluorescence staining,the numbers of γ-H2AX foci at different time points from 1 to 24 h post-irradiation were observed using a confocal laser scanning microscope.The average numbers of γ-H2AX foci observed were corrected using the Dolphin's model,followed by the fitting of dose-response curves.Using the R software,the equations and surfaces of the three-dimensional model for partial-body irradiation were established using the irradiation doses,post-irradiation time,and the corrected average numbers of γ-H2AX foci.Results The average number of γ-H2AX foci increased with dose at fixed time points 1,6,and 24 h but decreased with irradiation time at fixed doses 2,4,6,and 8 Gy.The dose-response curve equations of partial-body irradiation were fitted as follows:YF=2.853+3.775 D,R2=0.928,at 1 h after the irradiation;YF=0.144+2.775 D,R2=0.903,at 6 h after the irradiation;YF=0.066+2.472 D,R2=0.85,at 24 h after the irradiation.The three-dimensional model equation fitted was YF=6.837 t-1.728+3.113 t-0.071 D,R2=0.897.Substituting different post-irradiation time points into the three-dimensional surface model appeared as a two-dimensional linear model.By substituting the number of γ-H2AX foci and irradiation time into the linear and the three-dimensional models,both models yielded relative deviations between the estimated and actual radiation doses of 30％or less.Conclusions The three-dimensional time-dose-response model,established by using the number of γ-H2AX foci to estimate partial-body irradiation doses,can be preliminarily applied for dose estimation at all time points 1-24 h after irradiation.
γ-H2AXPartial-body irradiationDose estimationThree-dimensional model
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