骨桥蛋白在豚鼠胆囊胆固醇结石模型中的动态变化
Dynamic changes of osteopontin in the guinea pig model of cholesterol gallstone disease
陈进宏 1杨林 2林晶 1蔡建珊 1蔡端1
作者信息
- 1. 200040 上海,复旦大学附属华山医院普外科
- 2. 西安交通大学医学院第一附属医院外科
- 折叠
摘要
目的 观察骨桥蛋白(OPN)在豚鼠胆囊胆固醇结石模型中的动态变化.方法 将44只豚鼠随机分为胆固醇致石组和对照组,于饲养后1周、2周、4周、6周、8周和10周分别处死.实时PCR测定OPN在胆囊和肝脏组织中的表达,酶联免疫吸附法(EHSA)测定OPN、黏蛋白、α1-酸性糖蛋白(AAP)和载脂蛋白A1(APOA1)在胆汁和血浆中的变化.结果 OPN mRNA在豚鼠肝脏和胆囊组织中的表达变化一致,逐渐增高至第6周达到高峰,随后又逐渐下降.致石组胆汁中OPN合量从第1周开始上升,至第6周达最大值(P＜0.05),随后OPN浓度逐渐下降,至第10周,OPN含量已降至基线水平(P＞0.05).血浆OPN浓度变化与胆汁一致,但血浆OPN高峰出现早(第4周),随即逐渐下降至基线水平(P＞0.05).豚鼠胆结石形成过程中,APOAI在胆汁和血浆中的变化与OPN类似,但其血浆峰值没有提前.黏蛋白和AAP在造模开始后即逐渐升高,从第4周开始明显增高,随后继续升高并维持在一个高水平.结论 OPN参与胆囊胆固醇结石形成的整个过程,OPN与其他成核活性因子可能具有相互作用.
Abstract
Objective To investigate the dynamic changes of osteopontin (OPN) in the guinea pig model of cholesterol gallstone disease.Methods Forty-four guinea pigs were randomly assigned to cholesterol gallstone group and control group.The animals were sequentially killed on Day 7,14,28,42,56 and 70 after operation.The expressions of OPN mRNA in gallbladder and liver tissues were detected by real-time PCR.The changes of OPN,mucin,α1-acid glycoprotein (AAP) and apolipoprotein A1 (APOA1) in bile and blood plasma were detected by ELISA kits.Results The expression of OPN mRNA in gallbladder and liver tissues increased gradually and reached the peak in the 6th week,and then decreased.The increased expression of OPN in bile in the gallstone group started from the 1st week and reached the peak in the 6th week (P ＜ 0.05),which gradually decreased to the baseline in the 10th week (P ＞ 0.05).The expressions of OPN in bile and blood demonstrated similar trends,while the peak time in blood samples was much earlier (4th week).The changes of APOA1 in bile and blood were similar to OPN,although there was no advanced peak value in blood.The levels of mucin and AAP in bile and blood increased after operation,and were kept at high level throughout the study.Conclusions OPN is involved in the whole process of cholesterol gallstone formation,which may be associated with other nucleation-active factors.
关键词
骨桥蛋白/胆结石/胆固醇/豚鼠Key words
Osteopontin/Cholelithiasis/Cholesterol/Guinea pig引用本文复制引用
基金项目
国家自然科学基金资助项目(81270536)
出版年
2014
NETL
NSTL
维普
万方数据