Objective To investigate the expression of nuclear matrix protein 4(NMP4)in hepato-cellular carcinoma(HCC),and its relationship with clinicopathological features and survival prognosis of patients.Methods The clinical data of 100 HCC patients who were treated with radical resection of liver cancer in the Department of Hepatobiliary Surgery of the Third Affiliated Hospital of Wenzhou Medical Uni-versity from July 1,2014 to July 1,2019 were retrospectively analyzed.There were 63 males and 37 females,aged(58.5±10.4)years old.Immunohistochemical method was used to detect the expression of NMP4 protein in HCC cancer tissue and the corresponding adjacent normal tissue.According to the expression of NMP4 in HCC tissues,100 patients were divided into two groups:the NMP4-positive expression group(n=62)and the NMP4-negative expression group(n=32).Univariate analysis was performed on the relation-ship between NMP4 expression and clinical pathological features as well as overall survival of HCC patients.Cox multivariate analysis was performed on the factors influencing postoperative prognosis of HCC patients.Results Immunohistochemistry results showed that NMP4 was primarily expressed in the nucleus,the posi-tive expression rate of NMP4 in HCC tissues was higher than that in adjacent non-cancerous tissues[62.0％(62/100)vs.8.0％(8/100)],and the difference was statistically significant(x2=2.12,P=0.003).Univariate analysis revealed that the overall survival of HCC patients was correlated with the degree of tumor differentiation,tumor length,BCLC stage,number of tumor foci,vascular tumor thrombus and expression of NMP4(all P＜0.05).Cox multivariate analysis revealed that low differentiation,high BCLC stage(stage C),number of tumor foci(≥3),and positive expression of NMP4 were independent risk factors affecting postoperative survival and recurrence-free survival of HCC patients.The median overall survival and median recurrence-free survival of HCC patients in the NMP4-positive expression group were 22.3 months and 11.5 months,respectively.In contrast,that in the NMP4-negative expression group were 40.6 months and 19.4 months,respectively.The cumulative survival rate and recurrence-free survival rate of HCC patients in the NMP4-positive expression group were lower than those in the NMP4-negative expression group,and the differences were statistically significant(both P＜0.05).Conclusion Positive NMP4 expression was closely correlated with malignant biological progression and poor prognosis of HCC patients.
Carcinoma,hepatocellularNuclear matrix protein 4ImmunohistochemistryPrognosis
NETL
NSTL
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