Objective To analyze the expression of sequence similarity family 3 member C(FAM3C)in pancreatic cancer and its effects on pancreatic cancer cell proliferation,migration,invasion and the underlying mechanisms.Methods Tissue samples from four pancreatic cancer patients undergoing surgical resection at Affiliated Hospital of Jiangnan University from July 2022 to June 2023 were collected.Expression of FAM3C was assessed in both cancer and adjacent tissues using polymerase chain reaction and Western blot techniques.High FAM3C-expressing pancreatic cancer cells PANC-1 and MIA PaCa-2 were selected for further experiments involving FAM3C-siRNA and methyltransferase-like protein 3(METTL3)-siRNA groups,alongside a negative control group.Cell counting,Transwell assays,and co-immunoprecipitation with methylation antibodies were utilized to evaluate the proliferation,migration,inva-sion,and methylation status of FAM3C.Results The relative expression of FAM3C mRNA in cancer tissues of the four patients with pancreatic cancer was higher than that in adjacent tissues(1.29±0.19 vs 0.47±0.17,t=-6.48,P=0.001).Compared with the negative control group A,the proliferation ability of the FAM3C-siRNA interference group decreased,and the number of migrating and invasive cells decreased(all P＜0.05).In the negative control group B of PANC-1 cells,the relative expression of FAM3C mRNA in IgG-treated cells was lower than that in the methylated antibody-treated group(1.05±0.53 vs 30.57±1.09,t=-42.04,P=0.001).After PANC-1 cells were treated with methylated antibod-ies,the relative expression of FAM3C mRNA in the negative control group B was higher than that in the METTL3-siRNA interference group(30.57±1.09 vs 18.17±0.50,t=17.89,P=0.001).Compared with the negative control group B,the proliferation ability,migration and invasion cell numbers of PANC-1 cells in the METTL3-siRNA interference group were reduced(all P＜0.05).The results of MIA PaCa-2 and PANC-1 cells were consistent.Conclusions FAM3C is highly expressed in pancreatic cancer and promotes the proliferation,migration and invasion of pancreatic cancer cells.METTL3 affects FAM3C expression by methylation and proliferation,migration and invasion of pancreatic cancer cells.
Pancreatic neoplasmsMethyltransferase-like protein 3Family with sequence sim-ilarity 3 member CMigrationMetastasisProliferation
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