Objective To explore the feasibility of circulating tumor cell(CTC)dual-antibody enrichment and dual-antibody detection for epithelial-mesenchymal phenotypes in patients with hepatocellular carcinoma(HCC),and investigate the clinical diagnostic value of CTC typing in evaluating postoperative re-currence and prognosis of HCC.Methods Of 89 HCC patients who underwent surgical treatment in Zhe-jiang Jinhua Guangfu Tumor Hospital from March 2020 to January 2024 were enrolled into this study,inclu-ding 73 males and 16 females,aged(64.4±9.5)years old.The peripheral blood samples of patients were collected before operation.Epithelial CTC,mesenchymal CTC and hybrid epithelial-mesenchymal CTC in blood samples of patients with HCC were enriched and detected by EpCAM/CSV double capture antibodies and PanCK/CSV double detection antibodies.Kaplan-Meier analysis was employed to assess recurrence-free survival(RFS)and overall survival(OS)rates.Univariate and multivariate Cox regression were used to an-alyze the effects of different types of CTC on postoperative RFS and OS.Results The detection rates of total CTC,epithelial CTC,mesenchymal CTC and hybrid epithelial-mesenchymal CTC were 92.1％(82/89),64.0％(57/89),62.9％(56/89)and 55.1％(49/89),respectively.Multivariate Cox regression analy-sis showed that HCC patients with more mesenchymal CTC(HR=2.408,95％CI:1.580-3.668)and hy-brid epithelial-mesenchymal CTC(HR=1.840,95％CI:1.004-3.371)in peripheral blood had higher postoperative recurrence risk(both P＜0.05).Univariate Cox regression showed patients with more total CTC(HR=1.426,95％CI:1.040-1.954,P=0.028)was associated with survival.Conclusion The technique of epithelial-mesenchymal phenotypes analysis of circulating tumor cells based on dual-antibody capture and dual-antibody detection is feasible.The number of mesenchymal CTC and hybrid epithelial-mesenchymal CTC before operation is the influencing factor of postoperative recurrence in patients with hepa-tocellular carcinoma.
维普
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