中华肝胆外科杂志2024，Vol.30Issue(12) ：939-944.DOI:10.3760/cma.j.cn113884-20240516-00144

EB病毒核抗原1结合蛋白2对肝癌细胞的影响

Effect of Epstein-Barr virus nuclear antigen 1-binding protein 2 on the proliferation,invasion and migration of hepatocellular carcinoma cells

李燕军 李瑞 王壮强
中华肝胆外科杂志2024，Vol.30Issue(12) ：939-944.DOI:10.3760/cma.j.cn113884-20240516-00144
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EB病毒核抗原1结合蛋白2对肝癌细胞的影响

Effect of Epstein-Barr virus nuclear antigen 1-binding protein 2 on the proliferation,invasion and migration of hepatocellular carcinoma cells

李燕军 1李瑞 1王壮强1
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作者信息

  • 1. 山西医科大学第三医院 山西白求恩医院 山西医学科学院 同济山西医院肝胆外科,太原 030032
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摘要

目的 探讨EB病毒核抗原1结合蛋白2(EBP2)在肝细胞癌(HCC)组织中的表达水平,并分析EBP2对肝癌细胞增殖、侵袭和迁移能力的影响.方法 获取来自癌症基因组图谱数据库的HCC患者mRNA表达数据及相应的临床信息,共涵盖371例HCC样本和50例癌旁正常肝组织样本.分析EBP2基因的表达水平.设计针对EBP2的短发夹RNA(shRNA)序列,将他们克隆到慢病毒载体质粒中,作为敲减质粒;同时构建含有非特异性shRNA的阴性对照质粒.将这些质粒分别转染SMMC7721 和 BEL-7404 细胞,用于构建 EBP2 敲减组细胞(SMMC7721sh-EBP2组、BEL-7404sh-EBP2组)和各自的阴性对照组细胞(SMMC7221sh-Ctrl组、BEL-7404sh-Ctrl组).采用细胞计数实验、平板克隆形成实验、划痕愈合实验等检测EBP2基因敲减对肝癌细胞的影响.结果 EBP2在HCC肿瘤样本中的表达水平高于正常样本(2.256±0.312比1.362±0.341),差异具有统计学意义(t=7.85,P＜0.001).与SMMC7221sh-Ctrl组相比,SMMC7721shEBP2组肝癌细胞增殖活性(1.28±0.03比2.33±0.05)、形成集落的数量[(86.78±12.69)个比(194.54±14.82)个]、侵袭细胞数[(104.78±12.26)个比(224.18±9.57)个]以及迁移能力[(0.13±0.01)％比(0.24±0.01)％]均降低,差异均具有统计学意义(均P＜0.001).与 BEL-7404sh-Ctrl组相比,BEL-7404sh-EBP2组肝癌细胞活性(1.08±0.04 比 1.97±0.06)、形成集落的数量[(90.94±10.49)个比(185.36±11.03)个]、侵袭细胞数[(94.39±11.25)个比(198.66±10.14)个]以及迁移能力[(0.15±0.01)％比(0.38±0.01)％]均降低,差异均具有统计学意义(均P＜0.001).结论 EBP2在HCC癌组织中高表达,EBP2敲减可抑制肝癌细胞的增殖、迁移和侵袭能力.

Abstract

Objective To study the expression level of Epstein-Barr virus nuclear antigen 1-binding protein 2(EBP2)in hepatocellular carcinoma(HCC)tissues,and to analyze the effect of EBP2 on the pro-liferation,invasion and migration of HCC cells.Methods The mRNA expression data of HCC patients and the corresponding clinical information from the cancer genome atlas database were obtained,involving a total of 371 HCC samples and 50 adjacent normal liver tissue samples.The expression level of EBP2 gene was analyzed.Short hairpin RNA(shRNA)sequences targeting EBP2 were designed and cloned into lentiviral vector plasmids as knockdown plasmids;A negative control plasmid containing non-specific shRNA was con-structed at the same time.These plasmids were transfected into SMMC7721 and BEL-7404 cells,respectively,for the construction of EBP2 knockdown group cells(SMMC7721sh-EBP2 group,BEL-7404sh-EBP2 group)and their respective negative control group cells(SMMC7721sh-Ctrl group,BEL-7404sh-Ctrl group).Cell counting assays,plate clone formation assays,and scratch healing assays were used to detect the effects of EBP2 gene knockdown on HCC cells.Results The expression level of EBP2 in HCC tumor samples was higher than that in normal samples(2.256±0.312 vs.1.362±0.341,t=7.85,P＜0.001).Compared with the SMMC7221 shCtrl group,the proliferative activity of hepatoma cells(1.28±0.03 vs.2.33±0.05),the number of colonies formed(86.78±12.69 vs.194.54±14.82),the number of invasive cells(104.78±12.26 vs.224.18±9.57)and the migration ability[(0.13±0.01)％vs.(0.24±0.01)％]in the SMMC7221shEBP2 group were all reduced(all P＜0.001).Compared with the BEL-7404sh-Ctrl group,the HCC cell activity(1.08±0.04 vs.1.97±0.06),the number of colonies formed(90.94±10.49 vs.185.36±11.03),the number of invasive cells(94.39±11.25 vs.198.66±10.14)and the migration ability[(0.15±0.01)％vs.(0.38±0.01)％]in the BEL-7404shEBP2 group were all reduced(all P＜0.001).Conclusion EBP2 is highly expressed in HCC cancer tissues,and EBP2 knockdown bear the potential to inhibit the proliferation,migration and invasion of hepatocellular carcinoma cells.

关键词

癌,肝细胞/EB病毒核抗原1结合蛋白2/增殖/侵袭/迁移

Key words

Carcinoma,hepatocellular/Epstein-Barr virus nuclear antigen 1-binding protein 2/Proliferation/Invasion/Migrate

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出版年

2024
中华肝胆外科杂志
中华医学会

中华肝胆外科杂志

CSTPCDCSCD北大核心
影响因子：1.846
ISSN：1007-8118
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