中华肝脏病杂志2024，Vol.32Issue(2) ：148-154.DOI:10.3760/cma.j.cn501113-20231107-00174

乙型肝炎病毒相关肝癌釉丛蛋白和KLF-5过表达及其临床病理特征

Overexpression of tuftelin and KLF-5 and its clinicopathological features in hepatitis B virus-related hepatocellular carcinoma

杨君伶 方蓉菲 谢群 邰伯军 姚登福 姚敏
中华肝脏病杂志2024，Vol.32Issue(2) ：148-154.DOI:10.3760/cma.j.cn501113-20231107-00174
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  • 万方数据

乙型肝炎病毒相关肝癌釉丛蛋白和KLF-5过表达及其临床病理特征

Overexpression of tuftelin and KLF-5 and its clinicopathological features in hepatitis B virus-related hepatocellular carcinoma

杨君伶 1方蓉菲 2谢群 3邰伯军 4姚登福 5姚敏1
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作者信息

  • 1. 南通大学附属医院临床医学研究中心和医学院免疫学系,南通 226001
  • 2. 南通大学附属医院消化内科,南通 226001
  • 3. 海安市人民医院感染性疾病科,海安 226600
  • 4. 南通大学附属医院感染性疾病科,南通 226001
  • 5. 南通大学附属医院临床医学研究中心和医学院免疫学系,南通 226001;南通大学附属医院消化内科,南通 226001
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摘要

目的 分析乙型肝炎病毒(HBV)相关肝细胞癌(HCC)组织中釉丛蛋白(TUFT1)和锌指蛋白家族Krüppel样因子5(KLF5)表达,评估其对HCC的临床价值.方法 依据The Cancer Genome Atlas(TCGA)数据库资料,比较癌及非癌组 KLF5 mRNA 及 TUFT1 mRNA转录状态,分析组间差异及其预后价值;经伦理委员会批准,收集术后肝癌及其配对癌周组织制作组织芯片,以免疫组织化学法验证KLF5和TUFT1表达及其细胞内定位;以免疫印迹法分析组织中表达及其临床病理学特征;以SPSS软件行单/多因素分析其与患者预后的关系.结果 依据TCGA资料,HCC组TUFT1或KLF5 mRNA转录水平均显著高于非癌组(P＜0.001).免疫组织化学检查和蛋白质印迹法证实人HCC组织TUFT1和KLF5过表达,主要定位于细胞质和细胞膜.HCC 组织中 TUFT1 阳性率为 87.1％(x2=18.563,P＜0.001),KLF5 阳性率为 95.2％(x2=96.435,P＜0.001),二者均显著高于癌旁组;在国际抗癌联盟的标准Ⅲ～Ⅳ期其表达强度均高于Ⅰ～Ⅱ期(P＜0.01).临床病理特征显示,二者异常均与患者HBV感染、瘤体大小、伴肝外转移、TNM分期及伴腹水显著相关.单因素分析均与瘤体大小、HBV感染和患者生存率相关;多因素分析均是HCC患者的独立预后因素.结论 TUFT1和KLF5可能均为HBV相关HCC诊断和预后具有临床价值的新标志物.

Abstract

Objective To analyze and evaluate the expressions and clinical value of tuftelin(TUFT1)and Krüppel-like factor 5(KLF5)in hepatitis B virus(HBV)-related hepatocellular carcinoma(HCC)tissues.Method KLF5 mRNA and TUFT1 mRNA transcriptional status in cancer and non-cancer groups were compared according to the Cancer Genome Atlas(TCGA)database.The differences and prognostic value between the groups were analyzed.Postoperative liver cancer and its paired pericancerous tissues,with the approval of the ethics committee,were collected to build tissue chips.The expression of KLF5 and TUFT1 and their intracellular localization were verified by immunohistochemistry.Tissue expression and clinicopathological characteristics were analyzed by immunoblotting.SPSS software was used to analyze the relationship between SPSS and patient prognosis.Results The transcription level of TUFT1 or KLF5 mRNA was significantly higher in the HCC group than the non-cancer group(P＜0.001),according to TCGA data.Immunohistochemistry and Western blotting examination confirmed the overexpression of TUFT1 and KLF5 in human HCC tissues,which were mainly localized in the cytoplasm and cell membrane.The positivity rates of TUFT1 and KLF5 were 87.1％(x2=18.563,P＜0.001)and 95.2％(x2=96.435,P＜0.001)in HCC tissues,and both were significantly higher than those in the adjacent group.The expression intensity was higher in stage Ⅲ-Ⅳ than stage Ⅰ-Ⅱ of the International Union Against Cancer standard(P＜0.01).The clinicopathological features showed that the abnormalities of the two were significantly related to HBV infection,tumor size,extrahepatic metastasis,TNM stage,and ascites.Univariate analysis was related to tumor size,HBV infection,and survival.Multivariate analysis was an independent prognostic factor for patients with HCC.Conclusion TUFT1 and KLF5 may both be novel markers possessing clinical value in the diagnosis and prognosis of HBV-related HCC.

关键词

肝细胞癌/乙型肝炎病毒/TUFT1/KLF5/临床病理学特征/预后

Key words

Hepatocellular carcinoma/Hepatitis B virus/TUFT1/KLF5/Clinicopathological features/Prognosis

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基金项目

国家自然科学基金(31872738)

国家自然科学基金(81673241)

南通市传染病防治联盟基金(202308001)

出版年

2024
中华肝脏病杂志
中华医学会

中华肝脏病杂志

CSTPCDCSCD北大核心
影响因子：1.625
ISSN：1007-3418
参考文献量19
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