Objective:To investigate the expression and function of DEP domain-containing protein 1B (DEPDC1B) in hepatocellular carcinoma (HCC).Methods:Public cancer databases (UALCAN, HPA) were searched to analyze the expression profile of DEPDC1B in HCC. RNAseq data (level 3) and corresponding clinical information of HCC were obtained from The Cancer Genome Atlas (TCGA) (https://portal.gdc.com). Kaplan-Meier survival analysis was performed using Log-rank test to compare the survival differences between two groups or among multiple groups. Prognostic value of DEPDC1B was assessed by using univariate and multivariate Cox analysis and the nomogram was established. The expression of DEPDC1B was inhibited by siRNA. The changes of cell invasion and migration were detected by Transwell chamber assay. Protein-protein interaction (PPI) of DEPDC1B was constructed by using STRING and the underlying mechanism in HCC was unraveled.Results:The expression level of DEPDC1B gene in HCC tissues was significantly higher than that in normal tissues (P<0.05). The positive expression rate of DEPDC1B protein in HCC was 70%, ranking the first among 20 common cancers. TCGA data analysis showed that the overall survival and disease-free survival of HCC patients with high DEPDC1B expression were poor (HR=1.673, 1.434; P<0.05), which could be utilized as an independent prognostic factor of HCC and effectively predict the survival rate of HCC patients. Transwell chamber assay revealed that siRNA could significantly inhibit the expression levels of DEPDC1Bb in MHCC97-H and Hep3B cells. After down-regulating the expression of DEPDC1B, the invasion and migration capabilities of tumor cells were weakened. PPI network showed that DEPDC1B was intimately associated with tumor signaling pathways, such as TsC/mTOR, RTK, RAS/MAPK, PI3K/AKT, estrogen receptor, androgen receptor, epithelial-mesenchymal transition, DNA damage response, cell cycle and apoptosis, etc.Conclusions:DEPDC1B is highly expressed in HCC. High expression of DEPDC1B is correlated with poor prognosis of HCC patients. Down-regulating the expression of DEPDC1B can weaken the invasion and migration capabilities of HCC cells.
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