Effect of hyperbaric oxygen therapy on pain behavior and activation of spinal glial cells in rats with neuropathic pain
Objective To investigate the effect of hyperbaric oxygen(HBO)therapy on the pain behavior and the activation of spinal glial cells in rats with neuropathic pain.Methods A total of 36 Sprague-Dawley rats were divided into sham group(S group),chronic constriction injury of sciatic nerve group(CCI group),CCI combined with hyperbaric oxygen therapy group(CCI+HBO group),according to the random number table method,with 12 rats in each group.In the CCI+HBO group,HBO therapy started from the first day after the operation,and had been performed consecutively for 10 days with one hour per day.The mechanical withdrawal threshold(MWT)and the thermal withdrawal latency(TWL)were measured at one day before CCI injury modeling and on days 1,3,7,and 10 after CCI injury modeling.After finishing the 10-day HBO therapy,the expression levels of glial fibrillary acidic protein(GFAP)and ionized calcium-binding adapter molecule 1(Iba-1)in L4-L5 spinal cord were observed by using immunofluorescence and RT-qPCR.Results On day 10 after CCI injury modeling,compared with the S group,significant decreases of MWT and TWL were observed in the CCI group,the expression levels of GFAP and Iba-1 in spinal cord were also up-regulated in the CCI group(P＜0.05).Compared with the CCI group,significant increases of MWT and TWL were observed in the CCI+HBO group,the expression levels of GFAP and Iba-1 in spinal cord were also down-regulated in the CCI+HBO group(P＜0.05 or P＜0.01).Conclusion HBO can relieve neuropathic pain caused by CCI injury,which might be achieved by inhibiting the activation of spinal glial cells.
NETL
NSTL
万方数据
