Study of proanthocyanidin promotes osteogenic differentiation of human periodontal ligament stem cells through the transcription factor EB-induced autophagy-lysosome pathway
Objective To investigate the mechanism of proanthocyanidin(PA)in regulating the osteogenic differentiation of human periodontal ligament stem cells(PDLSCs),and to explore the effects of PA on the expression and nuclear translocation of transcription factor EB(TFEB)and on the autophagy-lysosome pathway.Methods PDLSCs were divided into control group and PA group,which were subjected to RNA sequencing analysis(RNA Seq)to detect differentially expressed genes.The osteogenic differentiation ability and autophagy level were observed by real-time fluorescence quantitative PCR(RT-qPCR)analysis,alkaline phosphatase(ALP)staining and transmission electron microscope(TEM),respectively.Scratch assay and Transwell assay were used to detect the migration ability of PDLSCs.Lysotracker and immunofluorescence staining were used to detect the biogenesis of lysosomes.The total protein expression of transcription factor EB(TFEB)as well as that in cytoplasm and nucleus were detected by Western blotting.Confocal laser scanning microscope(CLSM)was used to observe the nuclear translocation of TFEB.The PDLSCs were treated with small interfering RNA(siRNA)technology to knock down the expression levels of TFEB gene with or without PA treatment.Western blotting was used to analyze the expressions of autophagy-related proteins Beclin1 and microtubule-associated protein 1 light chain 3(LC3B),as well as osteogenic-related proteins runt-related transcription factor 2(RUNX2),ALP,and osteocalcin in PDLSCs.Results Compared with the control group,the osteogenic-related and autophagy-related genes showed differential expression in PDLSCs after PA treatment(P＜0.05).The mRNA expression levels of osteogenic-related genes RUNX2(2.32±0.15)and collagen type Ⅰ alpha 1(COL1a1)(1.80± 0.18),as well as the autophagy related genes LC3B(1.87±0.08)and Beclin1(1.63±0.08)were significantly increased in the PA group,compared with the control group(1.01±0.16,1.00±0.10,1.00±0.07,1.00±0.06,respectively,all P＜0.01).Compared with the control group,the PA group had higher ALP activity,and more autophagosomes and autophagolysosomes observed by TEM.PA promoted the migration of PDLSCs(P＜0.05)and the increased number of lysosomes and the expression of lysosomal associated membrane protein 1(LAMP1).In the PA group,the relative expression level of total TFEB protein(1.49±0.07)and the nuclear/cytoplasmic expression of TFEB protein(1.52±0.12)were significantly higher than the control group(1.00±0.11,1.00±0.13,respectively)(t=6.43,P＜0.01;t=5.07,P＜0.01).The relative nuclear/cytoplasmic fluorescence intensity of TFEB in the PA group(0.79±0.09)was increased compared with the control group(0.11± 0.08)(t=8.32,P＜0.01).Knocking down TFEB significantly reduced the expression of TFEB(1.00± 0.15 vs 0.64±0.04),LAMP1(1.00±0.10 vs 0.69±0.09),Beclin1(1.00±0.05 vs 0.60±0.05),and LC3B Ⅱ/Ⅰ(1.00±0.06 vs 0.73±0.07)in PDLSCs(P＜0.05,P＜0.05,P＜0.01,P＜0.01).When TFEB gene was knocked down,the expression levels of Beclin1(1.05±0.11),LC3B Ⅱ/Ⅰ(1.02±0.09),RUNX2(1.04±0.10),ALP(1.04±0.16),and osteocalcin(1.03±0.15)proteins were significantly decreased in the PA group compared with the pre-knockdown period(1.28±0.03,1.44±0.11,1.38±0.11,1.62±0.11,1.65±0.17,respectively)(P＜0.05,P＜0.01,P＜0.05,P＜0.01,and P＜0.01,respectively).Conclusions PA promotes the osteogenic differentiation of PDLSCs through inducing the expression and nuclear translocation of TFEB and activating the autophagy-lysosome pathway.
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