Emodin enhances succinate dehydrogenase activity and oxidation in Cryptococcus neoformans
Objective:To explore the effects of emodin on succinate dehydrogenase (SDH) activity and oxidation in Cryptococcus neoformans (C. neoformans) and elaborate the new biological function of emodin, so as to improve the sensitivity of antibacterial drugs targeting SDH and alleviate the serious problem of drug resistance.Methods:The minimal inhibitory concentration of emodin against C. neoformans was detected by broth microdilution and MTT assay, and the changes of reactive oxygen species (ROS) and mitochondrial membrane potential of C. neoformans treated with emodin were detected. The expression of SDH mRNA was detected by qPCR, and the interaction between emodin and SDH was detected by surface plasmon resonance.Results:Emodin had no obvious inhibitory effects on C. neoformans, with an MIC＞256 μg/ml, but emodin at a concentration of 1~4 μg/ml was able to promote the SDH activity of C. neoformans. At 1 μg/ml, emodin significantly increased the ROS and SDH mRNA expression. Emodin can bind with SDH specifically.Conclusion:Emodin can promote the SDH activity and enhance ROS production in C. neoformans, which represents the new biological function of emodin.
万方数据
