Identification and analysis of a case of foot skin infection with Schwanella algae in Hainan Province
Objective:To isolate a strain of Shewanella algae from wound secretion culture of a patient with foot skin infection in Hainan Province in May 2022 and to analyze its antimicrobial resistance related genes and antimicrobial susceptibility.Methods:A strain of Shewanella algae was isolated from a patient with foot skin infection, identified by Gram staining, VITEK 2 Compat, and matrix-assisted laser desorption ionization-time of flight mass spectrometry, and analyzed by 16S rRNA sequencing, gyrB gene sequencing, and average nucleotide identity. The antimicrobial susceptibility test and detection of resistance related genes were then carried out.Results:Microscopic examination of the isolate revealed a Gram-negative bacillus. Since the clinical identification system has certain limitations, the 16S rRNA sequence, gyrB gene sequence, and whole genome sequence were analyzed. Finally, the strain was determined to be Shewanella algae.Conclusion:The clinical identification system combined with molecular biology methods are required for Shewanella algae identification. This study was the first to identify a strain of Shewanella algae in a clinical case on Hainan Island in China, which provides a comprehensive methodological reference for clinical microbiology laboratories to accurately identify rare zoonotic pathogens, and clinical staff in Hainan should strengthen the understanding and monitoring of clinical infection caused by Shewanella.
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