Effect of exogenous leptin on intestinal mucosal proliferation in rats with obstructive jaundice
Objective:To explore the effect of exogenous leptin on the proliferation of the intestinal mucosa in rats with obstructive jaundice.Methods:Obstructive jaundice was induced by surgery in 32 Wistar rats, which were then randomly divided into either a control group or a study group, with 16 rats in each group. The study group was given recombinant leptin (10 μg/kg, bid) by intraperitoneal injection after operation, while the control group was injected with equal volume of saline. Rats in each group were sacrificed at 5 days and 8 days, and then labeled as C5 group, C8 group, S5 group, and S8 group, respectively. The general conditions of the rats were observed. Intestinal mucosal tissues were stained with hematoxylin-eosin (HE), and the changes of intestinal mucosal morphology and damage were observed. Immunohistochemistry (IHC) was used to detect the expression of leptin, leptin receptor, and Ki67 in the intestinal mucosa.Results:The intestinal mucosal injury index was significantly lower in the control group than in the study group, while it was significantly higher in the C5 and S5 groups than in the C8 and S8 groups. The expression of leptin and leptin receptor in the control group was significantly lower than that in the study group, while it was significantly higher in the C5 and S5 groups than that in the C8 and S8 groups, respectively.Conclusion:Exogenous leptin has the effect of promoting the proliferation and mitigate the damage of intestinal mucosal cells in rats with obstructive jaundice.
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