Effects of SNHG1 gene silencing on proliferation, apoptosis, migration, and ferroptosis of bladder cancer cells
Objective:To observe the effects of SNHG1 gene silencing on the proliferation, apoptosis, migration, and ferroptosis of bladder cancer cells.Methods:The expression of SNHG1 in human bladder cancer cell lines T24 and 5637 was detected by qPCR. SNHG1 shRNA was constructed and transfected into T24 and 5637 cell lines, respectively. The cells were divided into either a control group (shCtrl) or knockdown group (shSNHG1). Cell proliferation was detected by CCK8 method. Cell apoptosis was detected by flow cytometry. The migration ability of cells was detected by transwell assay. The levels of active iron ion, reactive oxygen species (ROS), and lipid peroxide (LPO) in cells were detected by ELISA. The mRNA levels of PTEN, Akt, GPX4, ACSL4, Caspase3, and CyclinD1 were detected by qPCR.Results:Compared with immortalized human bladder epithelial SV-HUC-1 cells, the expression of SNHG1 was significantly up-regulated in T24 and 5637 cells (P＜0.001). Compared with the control group, the proliferation and migration of cells in the knockdown group were significantly inhibited (P＜0.001), and the levels of apoptosis and ferroptosis were significantly increased (P＜0.001 and P＜0.05, respectively). Compared with the control group, the mRNA expression levels of PTEN, ACSL4, and Caspase3 in the knockdown group were significantly increased (P＜0.001), while the mRNA expression levels of GPX4, Akt, and CyclinD1 were significantly decreased (P＜0.01 and P＜0.001).Conclusion:Silencing SNHG1 expression can inhibit the proliferation and migration of human bladder cancer cells, and promote their apoptosis and ferroptosis. The mechanism may be related to the induction of PTEN, ACSL4, and Caspase3, and the inhibition of GPX4, Akt, and CyclinD1 expression.
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